Rabbit anti-Human Irak2 Polyclonal Antibody | anti-Irak2 antibody

Irak2, CT (Irak2, Interleukin-1 receptor-associated kinase-like 2) (APC)

Cat. Num. AAA6310829

• Gene Names: Irak2; IRAK-2; AI649099; 6330415L08Rik
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Irak2; Polyclonal Antibody; CT (Irak2; Interleukin-1 receptor-associated kinase-like 2) (APC); Interleukin-1 receptor-associated kinase-like 2; anti-Irak2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Irak2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Irak2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593-622 amino acids from the C-terminal region of Mouse Irak2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Irak2 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the Irak2 antibody detected the Irak2 protein (arrow).)

Related Product Information for anti-Irak2 antibody

Irak2 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization (By similarity).

Product Categories/Family for anti-Irak2 antibody

Antibodies; Protein Kinases

References

Hou, J., et al. J. Immunol. 183(3):2150-2158(2009). Conner, J.R., et al. J. Exp. Med. 206(7):1615-1631(2009). Wan, Y., et al. J. Biol. Chem. 284(16):10367-10375(2009). Elzinga, B.M., et al. J. Biol. Chem. 284(3):1394-1409(2009). Kawagoe, T., et al. Nat. Immunol. 9(6):684-691(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 165905631
• NCBI GeneID: 108960
• NCBI Accession #: NP_001107025.1; NP_751893.3
• NCBI GenBank Nucleotide #: NM_001113553.1
• UniProt Accession #: Q8CFA1; Q3U3K2; Q3U7F5; Q3UFX2; Q5U404; Q6YBR8; Q6YBR9; Q6YBS0; Q6YBS1; Q8C5M0; Q8CC82; Q8CEA0
• Molecular Weight: 69,047 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase-like 2 isoform b
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 2
• NCBI Official Symbol: Irak2
• NCBI Official Synonym Symbols: IRAK-2; AI649099; 6330415L08Rik
• NCBI Protein Information: interleukin-1 receptor-associated kinase-like 2; mu-IRAK-2; interleukin-1 receptor-associated kinase 2c
• UniProt Protein Name: Interleukin-1 receptor-associated kinase-like 2
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: Irak2
• UniProt Synonym Gene Names: IRAK-2; mu-IRAK-2
• UniProt Entry Name: IRAK2_MOUSE

Uniprot Description

IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; IRAK family

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; ATP binding; protein kinase activity

Biological Process: regulation of apoptosis; inhibition of NF-kappaB transcription factor; negative regulation of transcription factor activity; protein amino acid autophosphorylation; activation of NF-kappaB-inducing kinase; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor