Membrane Cofactor Protein (MCP) Recombinant Protein | MCP recombinant protein

Recombinant Membrane Cofactor Protein (MCP)

Cat. Num. AAA2010889

• Gene Names: Cd46; Mcp
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Membrane Cofactor Protein (MCP); Recombinant Membrane Cofactor Protein (MCP); MCP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DEAY WNGYPPHCEK IYCLPPPKIK NGTHTLTDIN VFKYHEAVSY SCDPTPGPDK FSLVGTSMIF CAGHNTWSNS PPECKVVKCP NPVLQNGRLI SGAGEIFSYQ STVMFECLQG FYMEGSSMVI CSANNSWEPS IPKCLKGPRP THPTKPPVYN YTGYPSPREG IFSQELDAWI IALIVITSIV GVFILCLIVLRCFEHRKKTN VSAAR
• Sequence Length: 365

• Applicable Applications for MCP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asp157~Arg365 (Accession # O88174) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single-pass type I membrane protein.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6754660
• NCBI GeneID: 17221
• NCBI Accession #: NP_034908.1
• NCBI GenBank Nucleotide #: NM_010778.3
• UniProt Accession #: O88174; Q9R0R9
• Molecular Weight: 55.2kDa
• NCBI Official Full Name: membrane cofactor protein
• NCBI Official Synonym Full Names: CD46 antigen, complement regulatory protein
• NCBI Official Symbol: Cd46
• NCBI Official Synonym Symbols: Mcp
• NCBI Protein Information: membrane cofactor protein
• UniProt Protein Name: Membrane cofactor protein
• UniProt Gene Name: Cd46
• UniProt Synonym Gene Names: Mcp
• UniProt Entry Name: MCP_MOUSE

Uniprot Description

CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Cellular Component: Golgi apparatus; inner acrosomal membrane; focal adhesion; cell surface; membrane; basolateral plasma membrane; cytoplasm; integral to membrane; acrosome; extracellular region; cytoplasmic vesicle

Molecular Function: enzyme inhibitor activity; complement binding; cadherin binding; endopeptidase activity

Biological Process: positive regulation of memory T cell differentiation; positive regulation of regulatory T cell differentiation; regulation of Notch signaling pathway; negative regulation of complement activation; single fertilization; T cell mediated immunity; negative regulation of catalytic activity; interleukin-10 production; positive regulation of T cell proliferation; proteolysis; positive regulation of interleukin-10 production