Rabbit anti-Human, Mouse MEK1, MEK2 Polyclonal Antibody | anti-Map2k2 antibody

MEK1, MEK2, phosphorylated (Ser218/Ser222) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-acti

Cat. Num. AAA624018

• Gene Names: Map2k2; MK2; MEK2; Prkmk2; AA589381
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: MEK1; MEK2; Polyclonal Antibody; phosphorylated (Ser218/Ser222) (MAPK/ERK Kinase 1; MEK 1; Dual Specificity Mitogen-activated Protein Kinase Kinase 1; MAP Kinase Kinase 1; MAP2K1; MAPKK 1; MKK1; PRKMK1; ERK Activator Kinase 1 / MAPK/ERK Kinase 2; Dual Specificity Mitogen-acti; Anti -MEK1; anti-Map2k2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MEK 1 and MEK 2, when phosphorylated at serine 218 and 222. MEK 1 and MEK 2 are also known as MAP2K1 and MAP2K2, respectively. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-Map2k2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. Recognizes a band of ~45kD in Western Blots of serum stimulated NIH3T3 cell lysates.; Optimal dilution to be determined by the researcher
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated serine 218 and 222 within MEK1 and 2.
• Positive Control: NIH 3T3 cell lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Map2k2 antibody

MEK1 and 2 are related dual specificity protein kinases from the MAP kinase kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.

DDC catalyses the second reaction in the biosynthesis of catecholamines and serotonin. In the final stages of dopamine synthesis, this enzyme converts L-3,4-dihydroxyphenylalanine (L-DOPA) to dopamine. This pathway is of particular research interest since L-DOPA is regularly used to treat the symptoms of Parkinson's disease.

Product Categories/Family for anti-Map2k2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 545203
• NCBI GeneID: 26396
• UniProt Accession #: Q63932; Q9D7B0
• Molecular Weight: 44,402 Da
• NCBI Official Full Name: MEK2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: Map2k2
• NCBI Official Synonym Symbols: MK2; MEK2; Prkmk2; AA589381
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2; MEK 2; MAPKK 2; MAPK/ERK kinase 2; MAP kinase kinase 2; MAP kinase/Erk kinase; ERK activator kinase 2; mitogen activated protein kinase kinase 2; protein kinase, mitogen activated, kinase 2, p45
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k2
• UniProt Synonym Gene Names: Mek2; Mkk2; Prkmk2; MAP kinase kinase 2; MAPKK 2; MEK 2
• UniProt Entry Name: MP2K2_MOUSE

Uniprot Description

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: Protein kinase, STE; EC 2.7.12.2; Kinase, protein; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Cellular Component: peroxisomal membrane; Golgi apparatus; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; intercellular junction; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus

Molecular Function: nucleotide binding; protein kinase activity; PDZ domain binding; MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; kinase activity; protein complex scaffold; ATP binding

Biological Process: regulation of axon regeneration; regulation of stress-activated MAPK cascade; activation of MAPK activity; MAPKKK cascade; positive regulation of axonogenesis; protein amino acid phosphorylation; phosphorylation