Immunoblot (IB)
(IB: Untreated (Lanes 1 and 3) and B-Raf phosphorylated (Lanes 2 and 4) PC-12 cell lysate was probed with MBS604178 (1:5000, Lanes 1 and 2) or total anti-MEK1 (1:1000, Lanes 3 and 4). Arrow indicates MEK1 (~45kD).)
Rabbit MEK1, MEK2 Monoclonal Antibody | anti-Map2k2 antibody
MEK1, MEK2, phosphorylated (Ser218/222, Ser222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mit
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Western Blot: 1:2000-1:10,000. Detects phosphorylated MEK1/2 in RIPA lysates from PC-12 cells that had been treated with 5u of B-Raf for 30 minutes at 30 degree C. Untreated and B-Raf phosphorylated PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2865-10B (1:5000) or Pab Rb anti-Mouse MEK1, NT (Map Kinase Kinase) (M2865-07) (1:1000). Proteins were visualized using a goat anti-rabbit secondary IgG labeled with HRP and a chemiluminescence detection system.
Immunoblot (IB)
(IB: Untreated (Lanes 1 and 3) and B-Raf phosphorylated (Lanes 2 and 4) PC-12 cell lysate was probed with MBS604178 (1:5000, Lanes 1 and 2) or total anti-MEK1 (1:1000, Lanes 3 and 4). Arrow indicates MEK1 (~45kD).)
Immunoblot (IB)
(IB: Untreated (Lanes 1 and 3) and B-Raf phosphorylated (Lanes 2 and 4) PC-12 cell lysate was probed with MBS604178 (1:5000, Lanes 1 and 2) or total anti-MEK1 (1:1000, Lanes 3 and 4). Arrow indicates MEK1 (~45kD).)
NCBI and Uniprot Product Information
Uniprot Description
MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.
Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); Kinase, protein; EC 2.7.12.2; STE group; STE7 family
Cellular Component: peroxisomal membrane; Golgi apparatus; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; cell cortex; intercellular junction; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus
Molecular Function: nucleotide binding; PDZ domain binding; protein kinase activity; MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; kinase activity; ATP binding
Biological Process: regulation of axon regeneration; regulation of stress-activated MAPK cascade; activation of MAPK activity; MAPKKK cascade; positive regulation of axonogenesis; phosphorylation; protein amino acid phosphorylation
Research Articles on Map2k2
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Product Notes
The Map2k2 map2k2 (Catalog #AAA604178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MEK1, MEK2, phosphorylated (Ser218/222, Ser222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mit reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1, MEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:2000-1:10,000. Detects phosphorylated MEK1/2 in RIPA lysates from PC-12 cells that had been treated with 5u of B-Raf for 30 minutes at 30 degree C. Untreated and B-Raf phosphorylated PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2865-10B (1:5000) or Pab Rb anti-Mouse MEK1, NT (Map Kinase Kinase) (M2865-07) (1:1000). Proteins were visualized using a goat anti-rabbit secondary IgG labeled with HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the Map2k2 map2k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1, MEK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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