Rabbit MED11 Polyclonal Antibody | anti-MED11 antibody

MED11 Polyclonal Antibody

Cat. Num. AAA3006819

• Gene Names: MED11; HSPC296
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MED11; Polyclonal Antibody; MED11 Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 11; Mediator complex subunit 11; HSPC296; anti-MED11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MED11 polyclonal antibody detects endogenous levels of MED11 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 117

• Applicable Applications for anti-MED11 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MED11.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MED11 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-MED11 antibody

In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multiprotein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med11 (mediator complex subunit 11) is a 117 amino acid nuclear protein and component of the mediator complex. The gene encoding Med11 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1, 200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, and is associated with predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

NCBI and Uniprot Product Information

• NCBI GI #: 48717372
• NCBI GeneID: 400569
• NCBI Accession #: NP_001001683.1
• NCBI GenBank Nucleotide #: NP_001001683.1
• UniProt Accession #: Q9P086
• Molecular Weight: ~ 13kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 11 isoform a
• NCBI Official Synonym Full Names: mediator complex subunit 11
• NCBI Official Symbol: MED11
• NCBI Official Synonym Symbols: HSPC296
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 11
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 11
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED11
• UniProt Synonym Gene Names: HSPC296
• UniProt Entry Name: MED11_HUMAN

NCBI Description

MED11 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]