Rabbit MED29 Polyclonal Antibody | anti-MED29 antibody

MED29 Polyclonal Antibody

Cat. Num. AAA3006435

• Gene Names: MED29; IXL; MED2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MED29; Polyclonal Antibody; MED29 Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 29; Intersex-like protein; Mediator complex subunit 29; IXL; anti-MED29 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MED29 polyclonal antibody detects endogenous levels of MED29 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 200

• Applicable Applications for anti-MED29 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MED29.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MED29 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse brain tissue lysateLane3:Rat lung tissue lysate)

Related Product Information for anti-MED29 antibody

In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med29 (mediator complex subunit 29), also known as IXL (intersex-like), is a 200 amino acid nuclear protein and component of the mediator complex. Widely expressed in embryo and adult tissue, Med29 is considered a novel amplification target gene in pancreatic cancer and is encoded by a gene that maps to human chromosome 19q13.2. Chromosome 19 consists of over 63 million bases, houses approximately 1, 400 genes and is recognized for having the greatest gene density of the human chromosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 17512074
• NCBI GeneID: 55588
• UniProt Accession #: Q9NX70
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: MED29 protein
• NCBI Official Synonym Full Names: mediator complex subunit 29
• NCBI Official Symbol: MED29
• NCBI Official Synonym Symbols: IXL; MED2
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 29
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 29
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED29
• UniProt Synonym Gene Names: IXL
• UniProt Entry Name: MED29_HUMAN

NCBI Description

MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]