Western Blot (WB)
(Western Blot (WB) analysis of MED15 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)
Rabbit MED15 Polyclonal Antibody | anti-MED15 antibody
MED15 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
MED15; Polyclonal Antibody; MED15 Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 15; Activator-recruited cofactor 105 kDa component; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein; CTG7A; PCQAP; TIG1; TNRC7; anti-MED15 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MED15 polyclonal antibody detects endogenous levels of MED15 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-MED15 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human MED15.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of MED15 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of MED15 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)
Related Product Information for anti-MED15 antibody
In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The Mediator complex is one such multiprotein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med15 (Mediator of RNA polymerase II transcription subunit 15), also known as ARC105, CTG7A, PCQAP, TIG1 or TNRC7, is a 788 amino acid subunit of the Mediator complex that localizes to both the nucleus and the cytoplasm. Expressed ubiquitously with highest expression in placenta and blood, Med15 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in the control of lipid homeostatsis. The gene encoding Med15 is located in a region on chromosome 22 that is deleted in DiGeorge syndrome, suggesting that the loss of Med15 may be associated with this rare congenital disease. Due to alternative splicing events, Med15 is expressed as two isoforms.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 87kDa
Protein Family
Similar Products
Product Notes
The MED15 (Catalog #AAA3006433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED15 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MED15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MED15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
