Western Blot (WB)
(Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit MECR Polyclonal Antibody | anti-MECR antibody
MECR Antibody
Gene Names
MECR; NRBF1; CGI-63; FASN2B
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
MECR Antibody is affinity chromatography purified via peptide column.
Synonyms
MECR; Polyclonal Antibody; MECR Antibody; NRBF1; CGI-63; FASN2B; NBRF1; Trans-2-enoyl-CoA reductase; mitochondrial; Nuclear receptor-binding factor 1; HsNrbf-1; mitochondrial trans-2-enoyl-CoA reductase; anti-MECR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of MECR are known to exist; this antibody will detect both isoforms.
Purity/Purification
MECR Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
373
Applicable Applications for anti-MECR antibody
ELISA (EIA), Western Blot (WB)
Application Notes
MECR antibody can be used for detection of MECR by Western blot at 1 - 2 mug/mL.
Conjugate
Unconjugated
Immunogen
Rabbit polyclonal MECR antibody was raised against a 15 amino acid peptide near the carboxy terminus of human MECR.
Buffer
MECR Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
MECR antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of MECR in (A) human brain tissue, (B) mouse brain tissue, and (C) rat brain tissue lysate with MECR antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of MECR in (A) human brain tissue, (B) mouse brain tissue, and (C) rat brain tissue lysate with MECR antibody at 1 μg/mL.)
Related Product Information for anti-MECR antibody
MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 41 kDa
NCBI Official Full Name
trans-2-enoyl-CoA reductase, mitochondrial isoform a
NCBI Official Synonym Full Names
mitochondrial trans-2-enoyl-CoA reductase
NCBI Official Symbol
MECR
NCBI Official Synonym Symbols
NRBF1; CGI-63; FASN2B
NCBI Protein Information
trans-2-enoyl-CoA reductase, mitochondrial; nuclear receptor binding factor 1; mitochondrial 2-enoyl thioester reductase; homolog of yeast 2-enoyl thioester reductase
UniProt Protein Name
Trans-2-enoyl-CoA reductase, mitochondrial
UniProt Gene Name
MECR
UniProt Synonym Gene Names
NBRF1; HsNrbf-1; NRBF-1
UniProt Entry Name
MECR_HUMAN
Uniprot Description
MECR: Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids. Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.3.1.38; Oxidoreductase; Lipid Metabolism - fatty acid elongation in mitochondria
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: mitochondrion; nucleus
Molecular Function: zinc ion binding; trans-2-enoyl-CoA reductase (NADPH) activity
Biological Process: fatty acid metabolic process; fatty acid biosynthetic process
Research Articles on MECR
Similar Products
Product Notes
The MECR mecr (Catalog #AAA153654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MECR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MECR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). MECR antibody can be used for detection of MECR by Western blot at 1 - 2 mug/mL. Researchers should empirically determine the suitability of the MECR mecr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MECR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
