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SDS-Page

Leptin Receptor Recombinant Protein | LEPR recombinant protein

Recombinant Leptin Receptor (LEPR)

Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 90%
Synonyms
Leptin Receptor; Recombinant Leptin Receptor (LEPR); CD295; LEPR; OBR; LR; LEPR recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
PPL GLRMEVTDDGNLKISWDSQT KAPFPLQYQV KYLENSTIVR EAAEIVSDTS LLVDSVLPGSSYEVQVRSKR LDGSGVWSDW SLPQLFTTQD VMYFPPKILT SVGSNASFCCIYKNENQTIS SKQIVWWMNL AEKIP
Sequence Length
896
Applicable Applications for LEPR recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Predicted Molecular Mass
19.3kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Pro238~Pro375
Organism Species
Rat
Tag
two N-terminal Tags, His-tag and T7-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
96,042 Da
NCBI Official Full Name
leptin receptor
NCBI Official Synonym Full Names
leptin receptor
NCBI Official Symbol
LEPR
NCBI Official Synonym Symbols
OBR; OB-R; CD295; LEP-R; LEPRD
NCBI Protein Information
leptin receptor
UniProt Protein Name
Leptin receptor
Protein Family
UniProt Gene Name
LEPR
UniProt Synonym Gene Names
DB; OBR; LEP-R; OB-R
UniProt Entry Name
LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: extracellular region; integral to membrane; plasma membrane; receptor complex

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; cholesterol metabolic process; energy reserve metabolic process; glucose homeostasis; leptin-mediated signaling pathway; multicellular organismal development; negative regulation of autophagy; negative regulation of gluconeogenesis; negative regulation of hydrolase activity; regulation of bone remodeling; sexual reproduction; T cell differentiation

Disease: Leptin Receptor Deficiency

Research Articles on LEPR

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Product Notes

The LEPR lepr (Catalog #AAA2033336) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Leptin Receptor can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the LEPR lepr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPL GLRMEVTDDG NLKISWDSQT KAPFPLQYQV KYLENSTIVR EAAEIVSDTS LLVDSVLPGS SYEVQVRSKR LDGSGVWSDW SLPQLFTTQD VMYFPPKILT SVGSNASFCC IYKNENQTIS SKQIVWWMNL AEKIP. It is sometimes possible for the material contained within the vial of "Leptin Receptor, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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