SDS-Page
MECR recombinant protein
MECR, 54-373aa, Human, His tag, E Coli
Gene Names
MECR; ETR1; NRBF1; CGI-63; FASN2B; DYTOABG
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
MECR; 54-373aa; Human; His tag; E Coli; Trans-2-enoyl-CoA reductase mitochondrial; NRBF1; CGI-63; FASN2B; Trans-2-enoyl-CoA reductase; mitochondrial; HsNrbf1; CGI 63; Homolog of yeast 2 enoyl thioester reductase; NBRF 1; NBRF1; NRBF 1; Nuclear receptor binding factor 1; MECR recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M
Sequence Length
373
Applicable Applications for MECR recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for MECR recombinant protein
MECR (Mitochondrial trans-2-enoyl-CoA reductase), also known as NRBF1, catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH dependent manner with preference to medium chain length substrate. This protein has a role in the mitochondrial synthesis of fatty acids. Recombinant human MECR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for MECR recombinant protein
References
Scicluna EA., et al. (2010) Eur J Clin Microbiol Infect Dis. 29 (2):163-70.; Chen ZJ., et al. (2008) J Mol Biol. 379 (4):830-44.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36.9 kDa (341aa) confirmed by MALDI-TOF
NCBI Official Full Name
Mitochondrial trans-2-enoyl-CoA reductase
NCBI Official Synonym Full Names
mitochondrial trans-2-enoyl-CoA reductase
NCBI Official Symbol
MECR
NCBI Official Synonym Symbols
ETR1; NRBF1; CGI-63; FASN2B; DYTOABG
NCBI Protein Information
enoyl-[acyl-carrier-protein] reductase, mitochondrial; trans-2-enoyl-CoA reductase, mitochondrial
UniProt Protein Name
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
UniProt Gene Name
MECR
UniProt Synonym Gene Names
NBRF1; HsNrbf-1; NRBF-1
NCBI Description
The protein encoded by this gene is an oxidoreductase that catalyzes the last step in mitochondrial fatty acid synthesis. Defects in this gene are a cause of childhood-onset dystonia and optic atrophy. [provided by RefSeq, Mar 2017]
Uniprot Description
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Has a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16).
Research Articles on MECR
Similar Products
Product Notes
The MECR mecr (Catalog #AAA203411) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's MECR can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the MECR mecr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M. It is sometimes possible for the material contained within the vial of "MECR, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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