Rabbit MATK Polyclonal Antibody | anti-MATK antibody

MATK Polyclonal Antibody

Cat. Num. AAA3011647

• Gene Names: MATK; CHK; CTK; HYL; Lsk; HYLTK; HHYLTK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MATK; Polyclonal Antibody; MATK Polyclonal Antibody; Megakaryocyte-associated tyrosine-protein kinase; CSK homologous kinase; CHK; Hematopoietic consensus tyrosine-lacking kinase; Protein kinase HYL; Tyrosine-protein kinase CTK; CTK; HYL; anti-MATK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MATK polyclonal antibody detects endogenous levels of MATK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 508

• Applicable Applications for anti-MATK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human MATK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MATK polyclonal antibody)

Related Product Information for anti-MATK antibody

All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine, Y527 in the case of Src p60, which is highly phosphorylated in the inactive form of the enzyme, while phosphorylated to a much lesser extent when the enzyme is active. For instance, a mutant of c-Src, in which Y527 is replaced by phenylalanine, is transforming and displays 5 to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as the carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also down regulates Fyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites. A Csk-like protein-tyrosine kinase of mouse cell origin (Ctk), also designated Ntk, and its human homolog, Lsk, have also been described.

NCBI and Uniprot Product Information

• NCBI GI #: 21450842
• NCBI GeneID: 4145
• NCBI Accession #: NP_002369.2
• NCBI GenBank Nucleotide #: NP_002369.2
• UniProt Accession #: P42679
• Molecular Weight: ~ 56kDa
• NCBI Official Full Name: megakaryocyte-associated tyrosine-protein kinase isoform b
• NCBI Official Synonym Full Names: megakaryocyte-associated tyrosine kinase
• NCBI Official Symbol: MATK
• NCBI Official Synonym Symbols: CHK; CTK; HYL; Lsk; HYLTK; HHYLTK
• NCBI Protein Information: megakaryocyte-associated tyrosine-protein kinase
• UniProt Protein Name: Megakaryocyte-associated tyrosine-protein kinase
• Protein Family: Maturase
• UniProt Gene Name: MATK
• UniProt Synonym Gene Names: CTK; HYL; CHK
• UniProt Entry Name: MATK_HUMAN

NCBI Description

The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTK: a non-receptor tyrosine kinase. Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation. Expressed in cancerous but not normal breast tissue; interacts with activated ERBB2 receptor and is likely to inhibit proliferation.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.2; TK group; Csk family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extrinsic to internal side of plasma membrane; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell proliferation; cell migration; positive regulation of cell proliferation; morphogenesis of an epithelium; innate immune response; mesoderm development; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation