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SDS-Page

Leucine Rich Repeat Containing Protein 32 Recombinant Protein | LRRC32 recombinant protein

Recombinant Leucine Rich Repeat Containing Protein 32 (LRRC32)

Gene Names
LRRC32; GARP; D11S833E
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Leucine Rich Repeat Containing Protein 32; Recombinant Leucine Rich Repeat Containing Protein 32 (LRRC32); LRRC32 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
LTRLARHTF WGMPAMEQLD LHSNVLMDIE DGAFEALPQL THLNLSRNSL TCISDFSLQQ LQVLDLSCNS IETFQTAPES QAQFQLAWLD LRENKLLHFP DLAMFPRLIY LNVSNNLIQL PTGLPQDSED LHAPSEGWSA SLLSNPSRNA SIHPLSQLLN LDLSYNEIEL VPAGFLEHLT SLRFLNLSRN CLRSFEARRA GSLPCLVLLD LSHNVLEALE LGTKVLGSLK TLLLQDNTLRELPPY
Sequence Length
662
Applicable Applications for LRRC32 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
31.1kDa
Accurate Molecular Mass (KD)
31kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag, T7 tag
Organism Species
Rattus norvegicus (Rat)
Fragment
Leu162~Tyr405
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,979 Da
NCBI Official Full Name
leucine-rich repeat-containing protein 32
NCBI Official Synonym Full Names
leucine rich repeat containing 32
NCBI Official Symbol
LRRC32
NCBI Official Synonym Symbols
GARP; D11S833E
NCBI Protein Information
leucine-rich repeat-containing protein 32
UniProt Protein Name
Leucine-rich repeat-containing protein 32
UniProt Gene Name
LRRC32
UniProt Synonym Gene Names
D11S833E; GARP; GARP
UniProt Entry Name
LRC32_HUMAN

NCBI Description

This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq, Jul 2008]

Uniprot Description

LRRC32:

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.5-q14

Cellular Component: integral to plasma membrane

Biological Process: negative regulation of activated T cell proliferation; negative regulation of cytokine secretion

Research Articles on LRRC32

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Product Notes

The LRRC32 lrrc32 (Catalog #AAA2029404) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Leucine Rich Repeat Containing Protein 32 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the LRRC32 lrrc32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
LTRLARHTF WGMPAMEQLD LHSNVLMDIE DGAFEALPQL THLNLSRNSL TCISDFSLQQ LQVLDLSCNS IETFQTAPES QAQFQLAWLD LRENKLLHFP DLAMFPRLIY LNVSNNLIQL PTGLPQDSED LHAPSEGWSA SLLSNPSRNA SIHPLSQLLN LDLSYNEIEL VPAGFLEHLT SLRFLNLSRN CLRSFEARRA GSLPCLVLLD LSHNVLEALE LGTKVLGSLK TLLLQDNTLR ELPPY. It is sometimes possible for the material contained within the vial of "Leucine Rich Repeat Containing Protein 32, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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