Rabbit anti-Human, Mouse MAP4K3 / GLK Polyclonal Antibody | anti-MAP4K3 antibody

Rabbit Polyclonal to Mouse MAP4K3 / GLK

Cat. Num. AAA248868

• Gene Names: MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity Purified
• Synonyms: MAP4K3 / GLK; Polyclonal Antibody; Rabbit Polyclonal to Mouse MAP4K3 / GLK; Anti-MAP4K3 / GLK Antibody (aa500-529) IHC-plus; MAP4K3; MEK kinase kinase 3; RAB8IPL1; MEKKK 3; MEKKK3; GCK-like kinase; GLK; MAPKKKK3; Mouse MAP4K3; anti-MAP4K3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Mouse MAP4K3 / GLK
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 894

• Applicable Applications for anti-MAP4K3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000)
• Target Species: Mouse
• Immunogen Description: KLH-conjugated synthetic peptide from internal region of Mouse Map4k3.
• Immunogen Type: Synthetic peptide
• Immunogen: MAP4K3 / GLK antibody was raised against kLH-conjugated synthetic peptide from internal region of Mouse Map4k3.
• Antigen Modification: aa500-529
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-MAP4K3 / GLK antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Flow Cytometry (FC/FACS)

(Mouse Map4k3 Antibody (Center) flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Mouse Map4k3 Antibody (Center) Western blot of HepG2 cell line lysates (35 ug/lane). This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

Western Blot (WB)

(Mouse Map4k3 Antibody (Center) Western blot of mouse Neuro-2a cell line lysates (35 ug/lane). This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

Related Product Information for anti-MAP4K3 antibody

GLK, a KHS protein kinase, activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of MEKK1. The protein has a structure similar to other GCK (Group I) kinases and contains an endophilin consensus-binding sequence PPRPPPPR, which has been shown to bind the SH3 domain of endophilin I in rat. GLK is activated by UV radiation and the proinflammatory cytokine tumor necrosis factor (TNF)-alpha.

Product Categories/Family for anti-MAP4K3 antibody

Family: Protein Kinase; Subfamily: KHS

NCBI and Uniprot Product Information

• NCBI GI #: 15451902
• NCBI GeneID: 8491
• NCBI Accession #: NP_003609.2
• NCBI GenBank Nucleotide #: NM_003618.3
• UniProt Accession #: Q8IVH8; Q6IQ39; Q8IVH7; Q9UDM5; Q9Y6R5
• Molecular Weight: 98,954 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: MAP4K3
• NCBI Official Synonym Symbols: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3; MEK kinase kinase 3; MAPK/ERK kinase kinase kinase 3; germinal center kinase-like kinase; germinal center kinase-related protein kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K3
• UniProt Synonym Gene Names: RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, STE; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV