Rabbit anti-Human MAP4K3 Polyclonal Antibody | anti-MAP4K3 antibody

MAP4K3 Antibody

Cat. Num. AAA9200692

• Gene Names: MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MAP4K3; Polyclonal Antibody; MAP4K3 Antibody; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; RAB8IPL1; anti-MAP4K3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MAP4K3 antibody is generated from rabbits immunized with a recombinant protein of human MAP4K3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 873

• Applicable Applications for anti-MAP4K3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Recombinant Protein
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of MAP4K3 Antibody in HepG2 cell line lysates (35ug/lane). MAP4K3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MAP4K3 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAP4K3 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MAP4K3 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAP4K3 antibody

MAP4K3 encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).

Product Categories/Family for anti-MAP4K3 antibody

Cancer; Metabolism; Signal Transduction

References

Yan, L., et al. Mol. Cell 37(5):633-642(2010)
Ichikawa, S., et al. J. Bone Miner. Res. (2010) In press :
Lam, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):11978-11983(2009)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 394025669
• NCBI GeneID: 8491
• NCBI Accession #: NP_001257354.1; NP_003609.2
• NCBI GenBank Nucleotide #: NM_001270425.1
• UniProt Accession #: Q8IVH8; Q6IQ39; Q8IVH7; Q9UDM5; Q9Y6R5
• Molecular Weight: 101316
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: MAP4K3
• NCBI Official Synonym Symbols: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K3
• UniProt Synonym Gene Names: RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; Kinase, protein; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV