Rabbit anti-Human MAP4K3 Polyclonal Antibody | anti-MAP4K3 antibody

MAP4K3 (MAP4K3, RAB8IPL1, Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, MAPK/ERK kinase kinase kinase 3) (MaxLight 490)

Cat. Num. AAA6318710

• Gene Names: MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MAP4K3; Polyclonal Antibody; MAP4K3 (MAP4K3; RAB8IPL1; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; MAPK/ERK kinase kinase kinase 3) (MaxLight 490); MAPK/ERK kinase kinase kinase 3; anti-MAP4K3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP4K3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-MAP4K3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MAP4K3.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HepG2 cell line lysates (35ug/lane) usingMBS6003875. MAP4K3 (arrow) was detected usingMBS6003875.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue using MBS6003875 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003875 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells using MBS6003875 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAP4K3 antibody

MAP4K3 encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).

Product Categories/Family for anti-MAP4K3 antibody

Antibodies; Protein Kinases

References

1. Yan, L., et al. Mol. Cell 37(5):633-642(2010). 2. Ichikawa, S., et al. J. Bone Miner. Res. (2010). 3. Lam, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):11978-11983(2009). 4. Wu, C., et al. Proteomics 7(11):1775-1785(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 394025669
• NCBI GeneID: 8491
• NCBI Accession #: NP_001257354.1
• NCBI GenBank Nucleotide #: NM_001270425.1
• UniProt Accession #: Q8IVH8; Q6IQ39; Q8IVH7; Q9UDM5; Q9Y6R5
• Molecular Weight: 101,316 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: MAP4K3
• NCBI Official Synonym Symbols: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3; MEK kinase kinase 3; MAPK/ERK kinase kinase kinase 3; germinal center kinase-like kinase; germinal center kinase-related protein kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K3
• UniProt Synonym Gene Names: RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV