Rabbit anti-Human PTHB1 Polyclonal Antibody | anti-PTHB1 antibody

PTHB1, CT (BBS9, PTHB1, Protein PTHB1, Bardet-Biedl syndrome 9 protein, Parathyroid hormone-responsive B1 gene protein) (FITC)

Cat. Num. AAA6338780

• Gene Names: BBS9; B1; D1; C18; PTHB1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTHB1; Polyclonal Antibody; CT (BBS9; Protein PTHB1; Bardet-Biedl syndrome 9 protein; Parathyroid hormone-responsive B1 gene protein) (FITC); BBS9; Parathyroid hormone-responsive B1 gene protein; anti-PTHB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PTHB1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PTHB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-824 amino acids from the C-terminal region of human PTHB1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTHB1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PTHB1 antibody detected the PTHB1 protein (arrow).)

Related Product Information for anti-PTHB1 antibody

This gene is downregulated by parathyroid hormone in osteoblastic cells, and therefore, is thought to be involved in parathyroid hormone action in bones. The exact function of this gene has not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-PTHB1 antibody

Antibodies; Disease Markers

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Wang, M., et al. Stat Biopharm Res 1(4):424-430(2009). Kang, H., et al. Hum. Reprod. 23(6):1457-1465(2008). Nachury, M.V., et al. Cell 129(6):1201-1213(2007). Nishimura, D.Y., et al. Am. J. Hum. Genet. 77(6):1021-1033(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 75905801
• NCBI GeneID: 27241
• NCBI Accession #: NP_001028776.1
• NCBI GenBank Nucleotide #: NM_001033604.1
• UniProt Accession #: Q3SYG4; P78514; Q7KYS6; Q7KYS7; Q8N570; Q99844; Q99854; Q9Y699; Q9Y6A0; E9PDC9
• Molecular Weight: 98,683 Da
• NCBI Official Full Name: protein PTHB1 isoform 3
• NCBI Official Synonym Full Names: Bardet-Biedl syndrome 9
• NCBI Official Symbol: BBS9
• NCBI Official Synonym Symbols: B1; D1; C18; PTHB1
• NCBI Protein Information: protein PTHB1
• UniProt Protein Name: Protein PTHB1
• UniProt Gene Name: BBS9
• UniProt Synonym Gene Names: PTHB1

NCBI Description

This gene is downregulated by parathyroid hormone in osteoblastic cells, and therefore is thought to be involved in parathyroid hormone action in bones. The exact function of this gene has not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2017]

Uniprot Description

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization.