Mouse anti-Human MAP4K3 Monoclonal Antibody | anti-MAP4K3 antibody

MAP4K3 Antibody

Cat. Num. AAA9200084

• Gene Names: MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Synonyms: MAP4K3; Monoclonal Antibody; MAP4K3 Antibody; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; RAB8IPL1; anti-MAP4K3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM,k
• Clone Number: 219CT8.3.1
• Specificity: This MAP4K3 monoclonal antibody is generated from mouse immunized with MAP4K3 recombinant protein.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Length: 873

• Applicable Applications for anti-MAP4K3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:100~500; IHC~~1:100
• Antigen Type: Recombinant Protein
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from MCF-7 cell line, using MAP4K3. MBS9200084 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using MAP4K3 Antibody. MBS9200084 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysates from HepG2, MCF-7 cell line (from left to right), using MAP4K3 Antibody. MBS9200084 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

(MAP4K3 western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAP4K3 antibody detected the MAP4K3 protein (arrow).)

Related Product Information for anti-MAP4K3 antibody

This gene encodes a member of the Ste20 family of
serine/threonine protein kinases. The protein belongs to the
subfamily that consists of members, such as germinal center kinase
(GCK), that are characterized by an N-terminal catalytic domain and
C-terminal regulatory domain. The kinase activity of the encoded
protein can be stimulated by UV radiation and tumor necrosis
factor-alpha. The protein specifically activates the c-Jun
N-terminal kinase (JNK) signaling pathway. Evidence suggests that
it functions upstream of mitogen-activated protein kinase kinase
kinase 1 (MEKK1). This gene previously was referred to as
RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed
mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).

Product Categories/Family for anti-MAP4K3 antibody

Cancer; Metabolism; Signal Transduction

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yan, L., et al. Mol. Cell 37(5):633-642(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 394025669
• NCBI GeneID: 8491
• NCBI Accession #: NP_001257354.1; NP_003609.2
• NCBI GenBank Nucleotide #: NM_001270425.1
• UniProt Accession #: Q8IVH8; Q6IQ39; Q8IVH7; Q9UDM5; Q9Y6R5
• Molecular Weight: 101316
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: MAP4K3
• NCBI Official Synonym Symbols: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K3
• UniProt Synonym Gene Names: RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV