Mouse anti-General M13 Monoclonal Antibody | anti-M13 antibody

Anti-M13 Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8106042

• Reactivity: General
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: M13; Monoclonal Antibody; Anti-M13 Antibody (PE); Mouse Monoclonal; M13 Bacteriophage Antibody (PE); Mouse Mab; anti-M13 antibody

• Host: Mouse
• Reactivity: General
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 05
• Specificity: The antibody binds specifically to phage coat proteins of fd phage or M13 phage
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.03%ProClin300
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-M13 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: M13 Bacteriophage
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with M13 Bacteriophage. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography, and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!

Testing Data

(4-1BB-transfected CHO-K1 cells were incubated with M13 phage particles for 20 minutes, then detected with 10 uL phage M13-PE Ab. The cell line stained with a 4-1BB specific M13 phage antibody (filied profile). Compared to an irrelevant phage antibody (open profile).The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-M13 antibody

M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E Coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E Coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form.

References

Messing, J. et al., 1993, Methods Mol. Biol. 23: 9-22. Mori, K. et al., 1996, Antiviral Res. 31 (1-2): 79-86. Sidhu, S.S. et al., 2001, Biomol Eng. 18 (2): 57-63. Sitohy, M. et al., 2006, J Agric Food Chem. 54 (11): 3800-6. Khalil, A.S. et al., 2007, Proc Natl Acad Sci. USA. 104 (12): 4892-7.