Rabbit anti-Human PAD4 Polyclonal Antibody | anti-PAD4 antibody

PAD4, CT (Protein-arginine Deiminase Type IV, Protein-arginine Deiminase Type-4, Peptidylarginine Deiminase IV, HL-60 PAD, PADI4, PADI5, PDI5) (MaxLight 550)

Cat. Num. AAA6491720

• Gene Names: PADI4; PAD; PAD4; PDI4; PDI5; PADI5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PAD4; Polyclonal Antibody; CT (Protein-arginine Deiminase Type IV; Protein-arginine Deiminase Type-4; Peptidylarginine Deiminase IV; HL-60 PAD; PADI4; PADI5; PDI5) (MaxLight 550); anti-PAD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAD4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-PAD4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PAD4 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PAD4 Pab in 293 cell line lysate. PAD4(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PAD4 antibody

Antibodies; Enzymes

References

1. Nakayama-Hamada, M., et al., Biochem. Biophys. Res. Commun. 327(1):192-200 (2005). 2. Wang, Y., et al., Science 306(5694):279-283 (2004). 3. Arita, K., et al., Arthritis Rheum. 11(8):777-783 (2004). 4. Barton, A., et al., Arthritis Rheum. 50(4):1117-1121 (2004). 5. Suzuki, A., et al., Nat. Genet. 34(4):395-402 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 216548487
• NCBI GeneID: 23569
• NCBI Accession #: NP_036519
• NCBI GenBank Nucleotide #: NM_012387
• UniProt Accession #: Q9UM07; Q5VTZ8; Q70SX4; A8K392; B2RBW0
• Molecular Weight: 74,079 Da
• NCBI Official Full Name: protein-arginine deiminase type-4
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type IV
• NCBI Official Symbol: PADI4
• NCBI Official Synonym Symbols: PAD; PAD4; PDI4; PDI5; PADI5
• NCBI Protein Information: protein-arginine deiminase type-4; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase, type V; protein-arginine deiminase type IV
• UniProt Protein Name: Protein-arginine deiminase type-4
• Protein Family: Lipase
• UniProt Gene Name: PADI4
• UniProt Synonym Gene Names: PADI5; PDI5
• UniProt Entry Name: PADI4_HUMAN

Uniprot Description

PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family.

Protein type: Nuclear receptor co-regulator; Hydrolase; EC 3.5.3.15

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm; nucleus

Molecular Function: arginine deiminase activity; protein binding; calcium ion binding; protein-arginine deiminase activity

Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; stem cell maintenance; innate immune response; protein modification process; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine

Disease: Rheumatoid Arthritis