Rabbit anti-Human MAGED1 Polyclonal Antibody | anti-MAGED1 antibody

MAGED1, ID (Melanoma-associated Antigen D1, MAGE-D1 Antigen, Neurotrophin Receptor-interacting MAGE Homolog, MAGE Tumor Antigen CCF, NRAGE, PRO2292, PP2250) (APC)

Cat. Num. AAA6486199

• Gene Names: MAGED1; NRAGE; DLXIN-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAGED1; Polyclonal Antibody; ID (Melanoma-associated Antigen D1; MAGE-D1 Antigen; Neurotrophin Receptor-interacting MAGE Homolog; MAGE Tumor Antigen CCF; NRAGE; PRO2292; PP2250) (APC); anti-MAGED1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGED1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAGED1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human MAGED1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MAGED1 Ctr Antibody is used in Western blot to detect MAGED1 in A549 lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MAGED1 antibody

Antibodies; Cancer Markers

References

1. Sasaki, A., et al., J. Biol. Chem. 277(25):22541-22546 (2002). 2. Kubu, C.J., et al., Genomics 70(1):150-152 (2000). 3. Salehi, A.H., et al., Neuron 27(2):279-288 (2000). 4. Pold, M., et al., Genomics 59(2):161-167 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 52632377
• NCBI GeneID: 9500
• NCBI Accession #: NP_008917
• NCBI GenBank Nucleotide #: NM_006986
• UniProt Accession #: Q9Y5V3
• Molecular Weight: 86kDa
• NCBI Official Full Name: melanoma-associated antigen D1 isoform b
• NCBI Official Synonym Full Names: MAGE family member D1
• NCBI Official Symbol: MAGED1
• NCBI Official Synonym Symbols: NRAGE; DLXIN-1
• NCBI Protein Information: melanoma-associated antigen D1
• UniProt Protein Name: Melanoma-associated antigen D1
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGED1
• UniProt Synonym Gene Names: NRAGE
• UniProt Entry Name: MAGD1_HUMAN

NCBI Description

This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NRAGE: a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although NRAGE shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. Binds p75 neurotrophin receptor (p75NTR) and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: protein complex; cytoplasm; plasma membrane; chromatin; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; positive regulation of MAP kinase activity; regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; circadian regulation of gene expression; regulation of circadian rhythm; negative regulation of transcription, DNA-dependent; negative regulation of epithelial cell proliferation