Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using DAB2 antibody MBS768460 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)
Rabbit DAB2 Polyclonal Antibody | anti-DAB2 antibody
DAB2 Rabbit Polyclonal
Purity: > or =95% as determined by SDS-PAGE
Purity: > or =95% as determined by SDS-PAGE
IP : 1:1000-1:10000
IF : 1:20-1:200
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using DAB2 antibody MBS768460 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using DAB2 antibody MBS768460 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)
Testing Data
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Testing Data
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Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS768460 (DAB2 antibody) at dilution of 1:1000)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS768460 (DAB2 antibody) at dilution of 1:1000)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
DAB2: an adaptor protein that interacts with Grb2, myosin VI, SMAD2/3, DIP1/2, Dvl-3, the integrin betasubunit, and c-Src. Negatively regulates canonical Wnt signaling by stabilizing the beta-catenin degradation complex. A component of the CSF-1 signal transduction pathway. Phosphorylated DAB2 inhibits adhesion and integrin signaling. Two alternatively spliced isoforms are described.
Protein type: Tumor suppressor; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 5p13.1
Cellular Component: clathrin coated vesicle membrane; clathrin-coated vesicle; coated pit; focal adhesion; intracellular membrane-bound organelle; lysosomal membrane; nucleolus; plasma membrane
Molecular Function: protein binding; protein C-terminus binding; SMAD binding
Biological Process: apoptosis; cell proliferation; integrin-mediated signaling pathway; leading edge cell differentiation; multicellular organismal development; negative regulation of apoptosis; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; positive regulation of endocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; protein transport; receptor-mediated endocytosis; Wnt receptor signaling pathway
Research Articles on DAB2
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Product Notes
The DAB2 dab2 (Catalog #AAA768460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAB2 Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DAB2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB : 1:500-1:5000 IP : 1:1000-1:10000 IF : 1:20-1:200. Researchers should empirically determine the suitability of the DAB2 dab2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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