Human Complexin 2 (CPLX2) ELISA Kit | CPLX2 elisa kit

Human Complexin 2 (CPLX2) ELISA Kit

Cat. Num. AAA2020555

• Gene Names: CPLX2; CPX2; Hfb1; 921-L; CPX-2
• Reactivity: Human
• Synonyms: Complexin 2 (CPLX2); Human Complexin 2 (CPLX2) ELISA Kit; 921-L; CPX-2; CPX2; Synaphin-1; CPLX2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complexin 2 (CPLX2).; No significant cross-reactivity or interference between Complexin 2 (CPLX2) and analogues was observed.
• Sequence Length: 114

• Assay Type: Competitive Inhibition
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 246.9-20000pg/mL
• Sensitivity: < 86.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complexin 2 (CPLX2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complexin 2 (CPLX2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPLX2 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complexin 2 (CPLX2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complexin 2 (CPLX2) and unlabeled Complexin 2 (CPLX2) (Standards or samples) with the pre-coated antibody specific to Complexin 2 (CPLX2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complexin 2 (CPLX2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complexin 2 (CPLX2) in the sample.

Product Categories/Family for CPLX2 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 46405548
• NCBI GeneID: 10814
• UniProt Accession #: Q6PUV4; O09056; Q13329; Q28184; Q52M15; Q64386; B2RAG2
• Molecular Weight: 15,394 Da
• NCBI Official Full Name: complexin 2, partial
• NCBI Official Synonym Full Names: complexin 2
• NCBI Official Symbol: CPLX2
• NCBI Official Synonym Symbols: CPX2; Hfb1; 921-L; CPX-2
• NCBI Protein Information: complexin-2
• UniProt Protein Name: Complexin-2
• Protein Family: Complexin
• UniProt Gene Name: CPLX2
• UniProt Synonym Gene Names: CPX II
• UniProt Entry Name: CPLX2_HUMAN

NCBI Description

Proteins encoded by the complexin/synaphin gene family are cytosolic proteins that function in synaptic vesicle exocytosis. These proteins bind syntaxin, part of the SNAP receptor. The protein product of this gene binds to the SNAP receptor complex and disrupts it, allowing transmitter release. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPLX2: Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis. Belongs to the complexin/synaphin family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: mast cell granule; cell soma; dendrite; synapse; cytosol

Molecular Function: syntaxin-1 binding; calcium-dependent protein binding

Biological Process: regulation of exocytosis; nervous system development; synaptic vesicle exocytosis; positive regulation of synaptic plasticity; mast cell degranulation; cell differentiation; vesicle docking during exocytosis