Western Blot (WB)
(Western blot analysis of extracts from HepG2, using MAGAC Antibody.)
Rabbit anti-Human MAGAC Polyclonal Antibody | anti-MAGAC antibody
MAGAC Antibody
Gene Names
MAGEA12; CT1.12; MAGE12
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MAGAC; Polyclonal Antibody; MAGAC Antibody; Cancer/testis antigen 1.12; Cancer/testis antigen family 1 member 12; CT1.12; MAGAC_HUMAN; MAGE 12 antigen; MAGE-12 antigen; MAGE12; MAGE12 antigen; MAGE12F; MAGE12F antigen; MAGEA12; Melanoma antigen family A 12; Melanoma associated antigen 12; Melanoma-associated antigen 12; anti-MAGAC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
MAGAC antibody detects endogenous levels of total MAGAC
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
314
Applicable Applications for anti-MAGAC antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human MAGAC
Tissue Specificity
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using MAGAC Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using MAGAC Antibody.)
Immunohistochemistry (IHC)
(MBS9602893 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602893 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of MAGAC using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of MAGAC using HeLa whole cell lysates)
Related Product Information for anti-MAGAC antibody
Function: Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 35 kDa
Predicted: 35 kDa
Predicted: 35 kDa
NCBI Official Full Name
melanoma-associated antigen 12
NCBI Official Synonym Full Names
MAGE family member A12
NCBI Official Symbol
MAGEA12
NCBI Official Synonym Symbols
CT1.12; MAGE12
NCBI Protein Information
melanoma-associated antigen 12
UniProt Protein Name
Melanoma-associated antigen 12
UniProt Gene Name
MAGEA12
UniProt Synonym Gene Names
MAGE12; CT1.12
NCBI Description
This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014]
Uniprot Description
Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines.
Research Articles on MAGAC
Similar Products
Product Notes
The MAGAC magea12 (Catalog #AAA9602893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGAC Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGAC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the MAGAC magea12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGAC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
