Rabbit Zap-70 Polyclonal Antibody | anti-Zap-70 antibody

Zap-70 (Phospho-Tyr493) Antibody

Cat. Num. AAA9408646

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Zap-70; Polyclonal Antibody; Zap-70 (Phospho-Tyr493) Antibody; 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70; anti-Zap-70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of Zap-70 only when phosphorylated at tyrosine 493.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 619

• Applicable Applications for anti-Zap-70 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 493 (S-Y-Y(p)-T-A) derived from Human Zap-70.
• Modification: Phospho-Tyr493
• Target Name: Zap-70
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from JK cells untreated or treated with anisomycin using Zap-70(Phospho-Tyr493) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Phospho-Tyr493) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-Zap-70 antibody

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Product Categories/Family for anti-Zap-70 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NM_001079.3
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 55,873 Da
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; protein amino acid phosphorylation; negative thymic T cell selection; positive regulation of T cell differentiation; innate immune response; beta selection; positive regulation of alpha-beta T cell proliferation; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive thymic T cell selection; T cell differentiation

Disease: Selective T-cell Defect