Rabbit anti-Human M11D1 Polyclonal Antibody | anti-M11D1 antibody

M11D1, NT (METTL17, METT11D1, Methyltransferase-like protein 17, mitochondrial, False p73 target gene protein, Methyltransferase 11 domain-containing protein 1, Protein RSM22 homolog, mitochondrial) (Biotin)

Cat. Num. AAA6317870

• Gene Names: METTL17; METT11D1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: M11D1; Polyclonal Antibody; NT (METTL17; METT11D1; Methyltransferase-like protein 17; mitochondrial; False p73 target gene protein; Methyltransferase 11 domain-containing protein 1; Protein RSM22 homolog; mitochondrial) (Biotin); METTL17; anti-M11D1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-M11D1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: M11D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 90-120 from the N-terminal region of human M11D1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of M11D1 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). M11D1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(M11D1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the M11D1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(M11D1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-M11D1 antibody

M11D1 may be a component of the mitochondrial small ribosomal subunit.

Product Categories/Family for anti-M11D1 antibody

Antibodies; Enzymes; Methyltransferase

References

Lim, J., et al. Cell 125(4):801-814(2006). Saveanu, C., et al. J. Biol. Chem. 276(19):15861-15867(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 71999135
• NCBI GeneID: 64745
• NCBI Accession #: NP_001025162.1
• NCBI GenBank Nucleotide #: NM_001029991.1
• UniProt Accession #: Q9H7H0; Q9BSH1; Q9BZH2; Q9BZH3
• Molecular Weight: 50,734 Da
• NCBI Official Full Name: methyltransferase-like protein 17, mitochondrial isoform 1
• NCBI Official Synonym Full Names: methyltransferase like 17
• NCBI Official Symbol: METTL17
• NCBI Official Synonym Symbols: METT11D1
• NCBI Protein Information: methyltransferase-like protein 17, mitochondrial; false p73 target gene protein; protein RSM22 homolog, mitochondrial; methyltransferase 11 domain containing 1; methyltransferase 11 domain-containing protein 1
• UniProt Protein Name: Methyltransferase-like protein 17, mitochondrial
• UniProt Gene Name: METTL17
• UniProt Synonym Gene Names: METT11D1
• UniProt Entry Name: MET17_HUMAN