Mouse CD14 ELISA Kit | CD14 elisa kit

Mouse CD14 PicoKine ELISA Kit

Cat. Num. AAA177179

• Reactivity: Mouse
• Synonyms: CD14; Mouse CD14 PicoKine ELISA Kit; Monocyte differentiation antigen CD14; CD14 molecule; CD 14; CD14 antigen; Myeloid cell-specific leucine-rich glycoprotein; CD14 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Cd14
• Sequence Length: 366

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 0.78 ng/ml - 50 ng/ml
• Sensitivity: <10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD14 elisa kit

Principle of the Assay: The Picokine™ Mouse Cd14 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Cd14 with a 96-well strip plate that is pre-coated with antibody specific for Cd14. The detection antibody is a biotinylated antibody specific for Cd14. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Cd14 with immunogen: Expression system for standard: NS0; Immunogen sequence: A18-P345. The kit is analytically validated with ready-to-use reagents. To measure Mouse Cd14, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Cd14 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cd14 in the sample.

Background/Introduction: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

NCBI and Uniprot Product Information

• NCBI GI #: 115957
• NCBI GeneID: 12475
• UniProt Accession #: P10810
• Molecular Weight: 39,204 Da
• NCBI Official Full Name: Monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 antigen
• NCBI Official Symbol: Cd14
• NCBI Protein Information: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: Cd14
• UniProt Entry Name: CD14_MOUSE

NCBI Description

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

Uniprot Description

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft

Molecular Function: lipopolysaccharide binding

Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of cytokine secretion