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Western Blot (WB) (All lanes : Anti-Kdm6a Antibody (C-term) at 1:2000 dilutionLane 1: mouse cerebellum lysatesLane 2: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 154 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Mouse Kdm6a Polyclonal Antibody | anti-Kdm6a antibody

Mouse Kdm6a Antibody (C-term)

Gene Names
Kdm6a; Utx
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Kdm6a; Polyclonal Antibody; Mouse Kdm6a Antibody (C-term); Lysine-specific demethylase 6A; 11411-; Histone demethylase UTX; Ubiquitously transcribed TPR protein on the X chromosome; Ubiquitously transcribed X chromosome tetratricopeptide repeat protein; Utx; anti-Kdm6a antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This mouse Kdm6a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1041-1074 amino acids from the C-terminal region of mouse Kdm6a.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1424
Applicable Applications for anti-Kdm6a antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Kdm6a Antibody (C-term) at 1:2000 dilutionLane 1: mouse cerebellum lysatesLane 2: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 154 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-Kdm6a Antibody (C-term) at 1:2000 dilutionLane 1: mouse cerebellum lysatesLane 2: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 154 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Kdm6a antibody
Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (By similarity).
Product Categories/Family for anti-Kdm6a antibody
References
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Greenfield A.,et al.Hum. Mol. Genet. 7:737-742(1998).
Grbavec D.,et al.Biochem. J. 337:13-17(1999).
Ballif B.A.,et al.Mol. Cell. Proteomics 3:1093-1101(2004).
Wang A.H.,et al.EMBO J. 32:1075-1086(2013).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
154355
NCBI Official Full Name
lysine-specific demethylase 6A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 6A
NCBI Official Symbol
Kdm6a
NCBI Official Synonym Symbols
Utx
NCBI Protein Information
lysine-specific demethylase 6A
UniProt Protein Name
Lysine-specific demethylase 6A
UniProt Gene Name
Kdm6a
UniProt Synonym Gene Names
Utx
UniProt Entry Name
KDM6A_MOUSE

Uniprot Description

UTX: a nuclear protein that contains 8 tetratrico peptide repeat (TPRs). Processes involving TPR proteins include cell-cycle control, transcription repression, stress response, protein kinase inhibition, mitochondrial and peroxisomal protein transport and neurogenesis.

Protein type: EC 1.14.11.-; Oxidoreductase

Cellular Component: histone methyltransferase complex; nucleus

Molecular Function: identical protein binding; procollagen-proline dioxygenase activity; protein binding; 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity; sulfonate dioxygenase activity; dioxygenase activity; metal ion binding; DNA-N1-methyladenine dioxygenase activity; oxidoreductase activity; chromatin binding

Biological Process: neural tube development; heart morphogenesis; in utero embryonic development; multicellular organism growth; heart development; mesodermal cell differentiation; chromatin modification; Wnt receptor signaling pathway through beta-catenin; somite rostral/caudal axis specification; respiratory system process; notochord morphogenesis; regulation of gene expression; neural tube closure; embryonic organ development

Research Articles on Kdm6a

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Product Notes

The Kdm6a kdm6a (Catalog #AAA9211920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Kdm6a Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Kdm6a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the Kdm6a kdm6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kdm6a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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