Rabbit LRRC8B Polyclonal Antibody | anti-LRRC8B antibody

Anti-LRRC8B Antibody, Rabbit Polyclonal

Cat. Num. AAA8102265

• Gene Names: LRRC8B; TALRRP; TA-LRRP
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: LRRC8B; Polyclonal Antibody; Anti-LRRC8B Antibody; Rabbit Polyclonal; LRRC8B Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-TA-LRRP Antibody; Anti-TALRRP Antibody; anti-LRRC8B antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LRRC8B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LRRC8B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human LRRC8B
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human LRRC8B, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-LRRC8B rabbit polyclonal antibody at 1:500 dilution Lane A: NCI-H292 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit  IgG (H&L) /Dylight 800  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:92 kDa Observed band size:92 kDa)

Immunoprecipitation (IP)

(LRRC8B was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-LRRC8B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LRRC8B rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 88 kDa Observed band size: 96 kDa)

Related Product Information for anti-LRRC8B antibody

Leucine-rich repeat-containing 8 (LRRC8) proteins are evolutionarily originated from the combination of the channel protein pannexin, and a leucine-rich repeat (LRR) domain. Five paralogs of LRRC8, namely LRRC8A, LRRC8B, LRRC8C, LRRC8D and LRRC8E have been reported. LRRC8A has been shown to be instrumental in cell swelling. LRRC8B is a key player in the cellular Ca2+ signaling network. LRRC8B participates in intracellular Ca2+ homeostasis by acting as a leak channel in the endoplasmic reticulum (ER).

References

Ghosh A, et al. (2017) Leucine-rich repeat-containing 8b protein is associated with the endoplasmic reticulum ca(2+) leak in hek293 cells. J Cell Sci 130 (22): 3818-3828.

NCBI and Uniprot Product Information

• NCBI GI #: 71296878
• NCBI GeneID: 23507
• UniProt Accession #: Q6P9F7; Q6UY21; Q8N106; Q92627; D3DT28
• Molecular Weight: 92,390 Da
• NCBI Official Full Name: LRRC8B protein
• NCBI Official Synonym Full Names: leucine rich repeat containing 8 family, member B
• NCBI Official Symbol: LRRC8B
• NCBI Official Synonym Symbols: TALRRP; TA-LRRP
• NCBI Protein Information: leucine-rich repeat-containing protein 8B; T cell activation leucine repeat rich protein; T-cell activation leucine repeat-rich protein
• UniProt Protein Name: Leucine-rich repeat-containing protein 8B
• Protein Family: Volume-regulated anion channel
• UniProt Gene Name: LRRC8B
• UniProt Synonym Gene Names: KIAA0231; TA-LRRP
• UniProt Entry Name: LRC8B_HUMAN

Uniprot Description

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Sequence similarities: Contains 13 LRR (leucine-rich) repeats.

Sequence caution: The sequence BAA13220.2 differs from that shown. Reason: Erroneous initiation.