Immunofluorescence (IF)
(Immunofluorescence staining of LRRC8B in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LRRC8B polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human LRRC8B Polyclonal Antibody | anti-LRRC8B antibody
Anti-LRRC8B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
LRRC8B; Polyclonal Antibody; Anti-LRRC8B Antibody; Rabbit Polyclonal; LRRC8B Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-TA-LRRP Antibody; Anti-TALRRP Antibody; anti-LRRC8B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human LRRC8B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-LRRC8B antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human LRRC8B fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human LRRC8B fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of LRRC8B in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LRRC8B polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of LRRC8B in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LRRC8B polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Related Product Information for anti-LRRC8B antibody
Leucine-rich repeat-containing 8 (LRRC8) proteins are evolutionarily originated from the combination of the channel protein pannexin, and a leucine-rich repeat (LRR) domain. Five paralogs of LRRC8, namely LRRC8A, LRRC8B, LRRC8C, LRRC8D and LRRC8E have been reported. LRRC8A has been shown to be instrumental in cell swelling. LRRC8B is a key player in the cellular Ca2+ signaling network. LRRC8B participates in intracellular Ca2+ homeostasis by acting as a leak channel in the endoplasmic reticulum (ER).
References
Ghosh A, et al. (2017) Leucine-rich repeat-containing 8b protein is associated with the endoplasmic reticulum ca(2+) leak in hek293 cells. J Cell Sci 130 (22): 3818-3828.
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Product Notes
The LRRC8B (Catalog #AAA8108661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LRRC8B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRRC8B can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the LRRC8B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRC8B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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