Rabbit anti-Mouse LRPAP1 Polyclonal Antibody | anti-LRPAP1 antibody

Anti-LRPAP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111321

• Gene Names: Lrpap1; RAP; HBP44; C77774; AA617339; AI790446; AU042172
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: LRPAP1; Polyclonal Antibody; Anti-LRPAP1 Antibody; Rabbit Polyclonal; LRPAP1 Antibody; Rabbit PAb; Antigen Affinity Purified; low density lipoprotein receptor-related protein associated protein 1; Anti-AA617339 Antibody; Anti-AI790446 Antibody; Anti-AU042172 Antibody; Anti-C77774 Antibody; Anti-HBP44 Antibody; Anti-RAP Antibody; anti-LRPAP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Acetylcholinesterase/ACHE
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LRPAP1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Mouse LRPAP1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Acetylcholinesterase/ACHE (rM Acetylcholinesterase/ACHE). Acetylcholinesterase/ACHE specific IgG was purified by Mouse Acetylcholinesterase/ACHE affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse LRPAP1 in mouse lung with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse LRPAP1 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(Mouse LRPAP1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-Mouse LRPAP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse LRPAP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 41 kDa)

Western Blot (WB)

(Anti-LRPAP1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: U87MG Whole Cell LysateLane C: PC3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)

Related Product Information for anti-LRPAP1 antibody

Receptor-associated protein (RAP) is a molecular chaperone for low-density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low-density lipoprotein receptor-related protein-associated protein 1, RAP, and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low-density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on the cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330) and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiasis. The genetic variations at the LRPAP1 locus may modulate Alzheimer's disease (AD) phenotype beyond risk for disease. Also, the variation in the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).

References

Gonzlez P, et al. (2002) Variation in the lipoprotein receptor-related protein, alpha2-macroglobulin and lipoprotein receptor-associated protein genes in relation to plasma lipid levels and risk of early myocardial infarction. Coron Artery Dis. 13(5): 251-4.Schutte DL, et al. (2003) A LRPAP1 intronic insertion/deletion polymorphism and phenotypic variability in Alzheimer disease. Res Theory Nurs Pract. 17(4): 301-19?Pandey SN, et al. (2006) Lipoprotein receptor associated protein (LRPAP1) insertion/deletion polymorphism: association with gallbladder cancer susceptibility. Int J Gastrointest Cancer. 37(4): 124-8.Dixit M, et al. (2006) Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.J Gastroenterol Hepatol. 21(5): 847-9.

NCBI and Uniprot Product Information

• NCBI GI #: 63999380
• NCBI GeneID: 16976
• NCBI Accession #: NP_038615.2
• NCBI GenBank Nucleotide #: NM_013587.2
• UniProt Accession #: P55302
• Molecular Weight: 42,215 Da
• NCBI Official Full Name: alpha-2-macroglobulin receptor-associated protein
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein associated protein 1
• NCBI Official Symbol: Lrpap1
• NCBI Official Synonym Symbols: RAP; HBP44; C77774; AA617339; AI790446; AU042172
• NCBI Protein Information: alpha-2-macroglobulin receptor-associated protein
• UniProt Protein Name: Alpha-2-macroglobulin receptor-associated protein
• Protein Family: Alpha-2-macroglobulin receptor-associated protein
• UniProt Gene Name: Lrpap1
• UniProt Synonym Gene Names: Alpha-2-MRAP; HBP-44; RAP
• UniProt Entry Name: AMRP_MOUSE

Uniprot Description

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Cellular Component: cytoplasm; endoplasmic reticulum; plasma membrane; rough endoplasmic reticulum; vesicle

Molecular Function: low-density lipoprotein receptor binding; receptor antagonist activity

Biological Process: negative regulation of protein binding