Rabbit anti-Human LRPAP1 Polyclonal Antibody | anti-LRPAP1 antibody

Anti-LRPAP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104877

• Gene Names: LRPAP1; RAP; MRAP; A2RAP; HBP44; A2MRAP
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: LRPAP1; Polyclonal Antibody; Anti-LRPAP1 Antibody; Rabbit Polyclonal; LRPAP1/A2MRAP Antibody; Rabbit PAb; Antigen Affinity Purified; low density lipoprotein receptor-related protein associated protein 1; Anti-A2MRAP Antibody; Anti-A2RAP Antibody; Anti-alpha-2-MRAP Antibody; Anti-HBP44 Antibody; Anti-MRAP Antibody; Anti-MYP23 Antibody; Anti-RAP Antibody; anti-LRPAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LRPAP1/A2MRAP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LRPAP1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:5000-1:10,000; IHC-P: 1:2000-1:10,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human LRPAP1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human LRPAP1/A2MRAP (rh LRPAP1). LRPAP1 specific IgG was purified by human LRPAP1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human LRPAP1 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human LRPAP1 in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human LRPAP1 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(LRPAP1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-LRPAP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LRPAP1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 41 kDa)

Western Blot (WB)

(Anti-LRPAP1 rabbit polyclonal antibody at 1:500 dilutionLane A: U87-MG Whole Cell LysateLane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:41 kDa)

Related Product Information for anti-LRPAP1 antibody

Receptor-associated protein (RAP) is a molecular chaperone for low-density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low-density lipoprotein receptor-related protein-associated protein 1, RAP, and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low-density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on the cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330) and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiasis. The genetic variations at the LRPAP1 locus may modulate Alzheimer's disease (AD) phenotype beyond risk for disease. Also, the variation in the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).

References

Gonzlez P, et al. (2002) Variation in the lipoprotein receptor-related protein, alpha2-macroglobulin and lipoprotein receptor-associated protein genes in relation to plasma lipid levels and risk of early myocardial infarction. Coron Artery Dis. 13(5): 251-4.Schutte DL, et al. (2003) A LRPAP1 intronic insertion/deletion polymorphism and phenotypic variability in Alzheimer disease. Res Theory Nurs Pract. 17(4): 301-19?Pandey SN, et al. (2006) Lipoprotein receptor associated protein (LRPAP1) insertion/deletion polymorphism: association with gallbladder cancer susceptibility. Int J Gastrointest Cancer. 37(4): 124-8.Dixit M, et al. (2006) Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.J Gastroenterol Hepatol. 21(5): 847-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4505021
• NCBI GeneID: 4043
• NCBI Accession #: NP_002328.1
• NCBI GenBank Nucleotide #: NM_002337.3
• UniProt Accession #: P30533; Q2M310; Q53HQ3; Q53HS6; D3DVR9
• Molecular Weight: 160,000
• NCBI Official Full Name: alpha-2-macroglobulin receptor-associated protein
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein associated protein 1
• NCBI Official Symbol: LRPAP1
• NCBI Official Synonym Symbols: RAP; MRAP; A2RAP; HBP44; A2MRAP
• NCBI Protein Information: alpha-2-macroglobulin receptor-associated protein; alpha-2-MRAP; lipoprotein receptor associated protein; alpha-2-macroglobulin receptor-associated protein 1; low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-r
• UniProt Protein Name: Alpha-2-macroglobulin receptor-associated protein
• Protein Family: Alpha-2-macroglobulin receptor-associated protein
• UniProt Gene Name: LRPAP1
• UniProt Synonym Gene Names: A2MRAP; Alpha-2-MRAP; RAP
• UniProt Entry Name: AMRP_HUMAN

NCBI Description

This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cell surface; endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane; vesicle

Molecular Function: heparin binding; receptor antagonist activity; protein binding; low-density lipoprotein receptor binding; unfolded protein binding; asialoglycoprotein receptor activity

Biological Process: vesicle-mediated transport; receptor-mediated endocytosis; protein folding; negative regulation of protein binding

Disease: Myopia 23, Autosomal Recessive