LRPAP1 / RAP recombinant protein

Recombinant Mouse LRPAP1 / RAP Protein (His tag)

Cat. Num. AAA2547057

• Gene Names: Lrpap1; RAP; HBP44; C77774; AA617339; AI790446; AU042172
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: LRPAP1 / RAP; Recombinant Mouse LRPAP1 / RAP Protein (His tag); AA617339; AI790446; AU042172; C77774; HBP44; RAP; LRPAP1 / RAP recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 360

• Application Notes: The recombinant mouse LRPAP1 comprises 343 amino acids with a predicted molecular mass of 40.4 kDa. As a result of glycosylation, it migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 29
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for LRPAP1 / RAP recombinant protein

Background: Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiosis. The genetic variations at the LRPAP1 locus may modulate Alzheimer disease (AD) phenotype beyond risk for disease. In addition, the variation at the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).

Description: A DNA sequence encoding the mature form of mouse LRPAP1 (NP_038615.2) extracellular domain (Gln 29-Leu 360) was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 63999380
• NCBI GeneID: 16976
• NCBI Accession #: NP_038615.2
• NCBI GenBank Nucleotide #: NM_013587.2
• UniProt Accession #: P55302
• Molecular Weight: 42,215 Da
• NCBI Official Full Name: alpha-2-macroglobulin receptor-associated protein
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein associated protein 1
• NCBI Official Symbol: Lrpap1
• NCBI Official Synonym Symbols: RAP; HBP44; C77774; AA617339; AI790446; AU042172
• NCBI Protein Information: alpha-2-macroglobulin receptor-associated protein
• UniProt Protein Name: Alpha-2-macroglobulin receptor-associated protein
• UniProt Gene Name: Lrpap1
• UniProt Synonym Gene Names: Alpha-2-MRAP; HBP-44; RAP

Uniprot Description

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 5 B2|5 18.01 cM

Cellular Component: cytoplasm; endoplasmic reticulum; plasma membrane; rough endoplasmic reticulum; vesicle

Molecular Function: low-density lipoprotein receptor binding; receptor antagonist activity

Biological Process: negative regulation of protein binding