Rabbit anti-Human LOXL3 Polyclonal Antibody | anti-LOXL3 antibody

LOXL3, CT (LOXL3, LOXL, Lysyl oxidase homolog 3, Lysyl oxidase-like protein 3) (APC)

Cat. Num. AAA6316758

• Gene Names: LOXL3; LOXL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LOXL3; Polyclonal Antibody; CT (LOXL3; LOXL; Lysyl oxidase homolog 3; Lysyl oxidase-like protein 3) (APC); Lysyl oxidase-like protein 3; anti-LOXL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-LOXL3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LOXL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 722-752 amino acids from the C-terminal region of human LOXL3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LOXL3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the LOXL3 antibody detected the LOXL3 protein (arrow).)

Immunohistochemistry (IHC)

(LOXL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LOXL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-LOXL3 antibody

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Alternatively spliced transcript variants of this gene have been reported but their full-length nature has not been determined.

Product Categories/Family for anti-LOXL3 antibody

Antibodies; Enzymes; Oxidase

References

Kim, Y., et al. Oncol. Rep. 22(4):799-804(2009). Sebban, S., et al. Virchows Arch. 454(1):71-79(2009). Akagawa, H., et al. Hum. Genet. 121 (3-4), 377-387 (2007): Lee, J.E., et al. J. Biol. Chem. 281(49):37282-37290(2006). Peinado, H., et al. EMBO J. 24(19):3446-3458(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 22095373
• NCBI GeneID: 84695
• NCBI Accession #: NP_115992.1
• NCBI GenBank Nucleotide #: NM_032603
• UniProt Accession #: P58215; Q96RS1; D6W5J1
• Molecular Weight: ~54 kDa
• NCBI Official Full Name: Homo sapiens lysyl oxidase-like 3 (LOXL3), mRNA
• NCBI Official Synonym Full Names: lysyl oxidase-like 3
• NCBI Official Symbol: LOXL3
• NCBI Official Synonym Symbols: LOXL
• NCBI Protein Information: lysyl oxidase homolog 3; lysyl oxidase-like protein 3
• UniProt Protein Name: Lysyl oxidase homolog 3
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL3
• UniProt Synonym Gene Names: LOXL
• UniProt Entry Name: LOXL3_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Alternatively spliced transcript variants of this gene have been reported but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL3: a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Alternatively spliced transcript variants of this gene have been reported but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Protein type: Oxidoreductase; EC 1.4.3.-; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: cytoplasm; extracellular region; extracellular space; membrane; nucleus

Molecular Function: copper ion binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity

Biological Process: epithelial to mesenchymal transition; negative regulation of transcription, DNA-dependent; receptor-mediated endocytosis