Western Blot (WB)
(Western blot analysis of HeLa cell using beta-tubulin antibody at different dilution. Each lane was loaded with 10 ug cell lysate.)
Rabbit beta-Tubulin Antibody | anti-beta-Tubulin antibody
beta-Tubulin Polyclonal Antibody
IHC 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of HeLa cell using beta-tubulin antibody at different dilution. Each lane was loaded with 10 ug cell lysate.)
Western Blot (WB)
(Western blot analysis of HeLa cell using beta-tubulin antibody at different dilution. Each lane was loaded with 10 ug cell lysate.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ?-Tubulin antibody (MBS125261) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ?-Tubulin antibody (MBS125261) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
NCBI and Uniprot Product Information
Calculated MW 50kDa
NCBI Description
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
Uniprot Description
TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: microtubule; cell soma; axon; dendrite; cytoplasm; nucleus
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; axon guidance; mitosis; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process
Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1
Research Articles on beta-Tubulin
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Product Notes
The beta-Tubulin tubb3 (Catalog #AAA125261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:5000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the beta-Tubulin tubb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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