LIFR active protein

LIFR Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122736

• Gene Names: Lifr; LIF; AW061234; A230075M04Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LIFR; LIFR Protein; Mouse; Recombinant (His Tag); Mouse LIFR/CD118 Protein (His Tag); A230075M04Rik Protein; AW061234 Protein; LIF Protein; leukemia inhibitory factor receptor alpha; LIFR active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser828

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 44
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse LIFR (NP_038612.1)(Met 1-Ser 828) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its ability to bind mouse LIF-Fc in a functional ELISA.; 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 5-20ng/mL in the presence of 2ng/mL recombinant human LIF.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LIFR active protein

Background: LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

References

Gearing, D.P. et al.,1991, EMBO J. 10 (10): 2839-2848. Gearing, D.P. et al.,1992, New Biol. 4 (1): 61-65. Mosley, B. et al.,1996, J. Biol. Chem. 271 (51): 32635-32643. Timmermann, A. et al.,2002, Eur. J. Biochem. 269 (11): 2716-2726. Lass, A. et al.,2002, Fertil. Steril. 76 (6): 1091-1096.

NCBI and Uniprot Product Information

• NCBI GI #: 7305235
• NCBI GeneID: 16880
• NCBI Accession #: NP_038612.1
• NCBI GenBank Nucleotide #: NM_013584.2
• UniProt Accession #: P42703; Q5I0Y2
• Molecular Weight: 81,287 Da
• NCBI Official Full Name: leukemia inhibitory factor receptor isoform 1
• NCBI Official Synonym Full Names: leukemia inhibitory factor receptor
• NCBI Official Symbol: Lifr
• NCBI Official Synonym Symbols: LIF; AW061234; A230075M04Rik
• NCBI Protein Information: leukemia inhibitory factor receptor
• UniProt Protein Name: Leukemia inhibitory factor receptor
• Protein Family: Leukemia inhibitory factor receptor
• UniProt Gene Name: Lifr
• UniProt Synonym Gene Names: LIF receptor; LIF-R

Uniprot Description

Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.