LIFR active protein

LIFR Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120391

• Gene Names: LIFR; SWS; SJS2; STWS; CD118; LIF-R
• Purity: >90% as determined by SDS-PAGE
• Synonyms: LIFR; LIFR Protein; Human; Recombinant (His Tag); Human LIFR/CD118 Protein (His Tag); CD118 Protein; LIF-R Protein; SJS2 Protein; STWS Protein; SWS Protein; leukemia inhibitory factor receptor alpha; LIFR active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser833

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 45
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human LIFR (NP_001121143.1)(Met 1-Ser 833) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to bind human LIF-Fc in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to bind human LIF-Fc in a functional ELISA.)

Related Product Information for LIFR active protein

Background: LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

References

Gearing, D.P. et al.,1991, EMBO J. 10 (10): 2839-2848. Gearing, D.P. et al.,1992, New Biol. 4 (1): 61-65. Mosley, B. et al.,1996, J. Biol. Chem. 271 (51): 32635-32643. Timmermann, A. et al.,2002, Eur. J. Biochem. 269 (11): 2716-2726. Lass, A. et al.,2002, Fertil. Steril. 76 (6): 1091-1096.

NCBI and Uniprot Product Information

• NCBI GI #: 189083786
• NCBI GeneID: 3977
• NCBI Accession #: NP_001121143.1
• NCBI GenBank Nucleotide #: NM_001127671.1
• UniProt Accession #: P42702; Q6LCD9
• Molecular Weight: 123,743 Da
• NCBI Official Full Name: leukemia inhibitory factor receptor
• NCBI Official Synonym Full Names: leukemia inhibitory factor receptor alpha
• NCBI Official Symbol: LIFR
• NCBI Official Synonym Symbols: SWS; SJS2; STWS; CD118; LIF-R
• NCBI Protein Information: leukemia inhibitory factor receptor; LIF receptor; CD118 antigen
• UniProt Protein Name: Leukemia inhibitory factor receptor
• Protein Family: Leukemia inhibitory factor receptor
• UniProt Gene Name: LIFR
• UniProt Synonym Gene Names: LIF receptor; LIF-R
• UniProt Entry Name: LIFR_HUMAN

NCBI Description

This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LIFR: Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells. Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS); also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue. A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: integral to plasma membrane; receptor complex

Molecular Function: oncostatin-M receptor activity; leukemia inhibitory factor receptor activity; growth factor binding; ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding

Biological Process: cell surface receptor linked signal transduction; response to cytokine stimulus; leukemia inhibitory factor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation

Disease: Stuve-wiedemann Syndrome