Rabbit anti-Mouse Leptin Receptor Polyclonal Antibody | anti-LEPR antibody

Anti-Leptin Receptor Antibody, Rabbit Polyclonal

Cat. Num. AAA8103534

• Gene Names: Lepr; db; Obr; obl; Leprb; Modb1; LEPROT; OB-RGRP; diabetes; obese-like
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Leptin Receptor; Polyclonal Antibody; Anti-Leptin Receptor Antibody; Rabbit Polyclonal; Leptin Receptor/LEPR Antibody; Rabbit PAb; Antigen Affinity Purified; leptin receptor; Anti-db Antibody; Anti-diabetes Antibody; Anti-Leprb Antibody; Anti-LEPROT Antibody; Anti-Modb1 Antibody; Anti-OB-RGRP Antibody; Anti-obese-like Antibody; Anti-obl Antibody; Anti-Obr Antibody; anti-LEPR antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Leptin Receptor/LEPR
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-LEPR antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Leptin Receptor/LEPR
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Leptin Receptor/LEPR, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-LEPR rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size:125 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse LEPR in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-LEPR antibody

Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight) and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra-Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K, and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in the active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in the Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.

References

Heshka JT, et al. (2001) A role for dietary fat in leptin receptor, OB-Rb, function. Life Sci. 69 (9): 987-1003.Chen H, et al (1996) Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell. 84 (3): 491-5.Bjrbaek C, et al. (1998) Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 272 (51): 32686-95.

NCBI and Uniprot Product Information

• NCBI GI #: 1352612
• NCBI GeneID: 16847
• UniProt Accession #: P48356; O35686; O54986; Q61215; Q64309; Q9QWG3; Q9QWV5
• Molecular Weight: 90,887 Da
• NCBI Official Full Name: Leptin receptor
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: Lepr
• NCBI Official Synonym Symbols: db; Obr; obl; Leprb; Modb1; LEPROT; OB-RGRP; diabetes; obese-like
• NCBI Protein Information: leptin receptor; B219; LEP-R; OB receptor; leptin receptor gene-related protein
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: Lepr
• UniProt Synonym Gene Names: Db; Obr; LEP-R; OB-R
• UniProt Entry Name: LEPR_MOUSE

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: extracellular space; membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity; peptide hormone binding; protein-hormone receptor activity

Biological Process: cholesterol metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of MAPKKK cascade; eating behavior; negative regulation of gluconeogenesis; negative regulation of hydrolase activity; regulation of metabolic process; angiogenesis; signal transduction; leptin-mediated signaling pathway