Mouse Leptin receptor ELISA Kit | LEPR elisa kit

Mouse Leptin receptor PicoKine ELISA Kit

Cat. Num. AAA175790

• Gene Names: Lepr; db; Obr; obl; Leprb; Modb1; LEPROT; OB-RGRP; diabetes; obese-like
• Reactivity: Mouse
• Synonyms: Leptin receptor; Mouse Leptin receptor PicoKine ELISA Kit; leptin receptor; CD295; HuB220; LEP-R; LEPR; LEPR_HUMAN; OB receptor; OB-R; OBR; LEPR elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Leptin receptor
• Sequence Length: 1162

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Leptin receptor PicoKine ELISA Kit standard curve )

Related Product Information for LEPR elisa kit

Principle of the assay: mouse Leptin receptor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Leptin receptor has been precoated onto 96-well plates. Standards(NSO,L22-G839) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin receptor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Leptin receptor amount of sample captured in plate.
Background: Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 1352612
• NCBI GeneID: 16847
• UniProt Accession #: P48356; O35686; O54986; Q61215; Q64309; Q9QWG3; Q9QWV5
• Molecular Weight: 90,887 Da
• NCBI Official Full Name: Leptin receptor
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: Lepr
• NCBI Official Synonym Symbols: db; Obr; obl; Leprb; Modb1; LEPROT; OB-RGRP; diabetes; obese-like
• NCBI Protein Information: leptin receptor; B219; LEP-R; OB receptor; leptin receptor gene-related protein
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: Lepr
• UniProt Synonym Gene Names: Db; Obr; LEP-R; OB-R
• UniProt Entry Name: LEPR_MOUSE

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: extracellular space; membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity; peptide hormone binding; protein-hormone receptor activity

Biological Process: cholesterol metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of MAPKKK cascade; eating behavior; negative regulation of gluconeogenesis; negative regulation of hydrolase activity; regulation of metabolic process; angiogenesis; signal transduction; leptin-mediated signaling pathway