Rabbit anti-Human LEO1 Polyclonal Antibody | anti-LEO1 antibody

LEO1, NT (RDL, RNA Polymerase-associated Protein LEO1, Replicative Senescence Down-regulated leo1-like Protein, RDL)

Cat. Num. AAA625172

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: LEO1; Polyclonal Antibody; NT (RDL; RNA Polymerase-associated Protein LEO1; Replicative Senescence Down-regulated leo1-like Protein; RDL); Anti -LEO1; anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LEO1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-LEO1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:250
• Immunogen: Synthetic peptide selected from the N-terminal region of human LEO1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Leo1 Antibody (N-term) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).)

Related Product Information for anti-LEO1 antibody

The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.

Product Categories/Family for anti-LEO1 antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 1420322
• UniProt Accession #: P38439; D6W2I1
• Molecular Weight: 53,920 Da
• NCBI Official Full Name: LEO1
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: LEO1
• UniProt Entry Name: LEO1_YEAST

Uniprot Description

Function: The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. Ref.9 Ref.10

Subunit structure: Component of the PAF1 complex which consists of at least CDC73, CTR9, LEO1, PAF1 and RTF1.

Subcellular location: Nucleus › nucleoplasm Ref.9.

Miscellaneous: Present with 1110 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the LEO1 family.