Western Blot (WB)
(Western blot analysis of lysates from mouse cerebellum, rat cerebellum tissue lysate (from left to right), using Leo1 Antibody (C-term). MBS9208960 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Mouse, rat Leo1 Polyclonal Antibody | anti-Leo1 antibody
Mouse Leo1 Antibody (C-term)
Gene Names
Leo1; Gm185
Reactivity
Mouse, rat
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Leo1; Polyclonal Antibody; Mouse Leo1 Antibody (C-term); RNA polymerase-associated protein LEO1; Gm185; anti-Leo1 antibody
Host
Rabbit
Reactivity
Mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Leo1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 616-650 amino acids from the C-terminal region of Mouse Leo1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
667
Applicable Applications for anti-Leo1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from mouse cerebellum, rat cerebellum tissue lysate (from left to right), using Leo1 Antibody (C-term). MBS9208960 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from mouse cerebellum, rat cerebellum tissue lysate (from left to right), using Leo1 Antibody (C-term). MBS9208960 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-Leo1 antibody
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity).
References
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Smith J.C.,et al.J. Proteome Res. 6:3174-3186(2007).
Villen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Ding L.,et al.Cell Stem Cell 4:403-415(2009).
Smith J.C.,et al.J. Proteome Res. 6:3174-3186(2007).
Villen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Ding L.,et al.Cell Stem Cell 4:403-415(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75597
NCBI Official Full Name
RNA polymerase-associated protein LEO1
NCBI Official Synonym Full Names
Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
NCBI Official Symbol
Leo1
NCBI Official Synonym Symbols
Gm185
NCBI Protein Information
RNA polymerase-associated protein LEO1
UniProt Protein Name
RNA polymerase-associated protein LEO1
Protein Family
UniProt Gene Name
Leo1
UniProt Synonym Gene Names
Gm185
UniProt Entry Name
LEO1_MOUSE
Uniprot Description
LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: protein binding
Biological Process: histone monoubiquitination; negative regulation of myeloid cell differentiation; Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; histone H2B ubiquitination; stem cell maintenance; positive regulation of mRNA 3'-end processing; mRNA polyadenylation; positive regulation of transcription from RNA polymerase II promoter
Similar Products
Product Notes
The Leo1 leo1 (Catalog #AAA9208960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Leo1 Antibody (C-term) reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Leo1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Leo1 leo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leo1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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