Rabbit anti-Human LEO1 Polyclonal Antibody | anti-LEO1 antibody

LEO1 Antibody

Cat. Num. AAA9412049

• Gene Names: LEO1; RDL
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: LEO1; Polyclonal Antibody; LEO1 Antibody; RDL; anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total LEO1 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.4 mg/ml (varies by lot)
• Sequence Length: 606

• Applicable Applications for anti-LEO1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:25-1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the N terminal of human Leo1, Paf1/RNA polymerase II complex component, homolog
• Target Name: LEO1
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)

Related Product Information for anti-LEO1 antibody

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex

Product Categories/Family for anti-LEO1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 556695389
• NCBI GeneID: 123169
• NCBI Accession #: NP_001273359.1
• NCBI GenBank Nucleotide #: NM_001286430.1
• UniProt Accession #: Q8WVC0; Q96N99
• Molecular Weight: 68,004 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1 isoform 2
• NCBI Official Synonym Full Names: LEO1 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: LEO1
• NCBI Official Synonym Symbols: RDL
• NCBI Protein Information: RNA polymerase-associated protein LEO1
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: LEO1
• UniProt Synonym Gene Names: RDL
• UniProt Entry Name: LEO1_HUMAN

NCBI Description

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]

Uniprot Description

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding

Biological Process: histone monoubiquitination; negative regulation of myeloid cell differentiation; Wnt receptor signaling pathway; transcription, DNA-dependent; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; stem cell maintenance; histone H2B ubiquitination; positive regulation of mRNA 3'-end processing; positive regulation of transcription from RNA polymerase II promoter; mRNA polyadenylation