Rat Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit | NOS2 elisa kit

Rat Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit

Cat. Num. AAA452452

• Gene Names: Nos2; iNos; Nos2a
• Reactivity: Rat
• Synonyms: Nitric Oxide Synthase 2; Inducible (NOS2); Rat Nitric Oxide Synthase 2; Inducible (NOS2) ELISA Kit; NOS2A INOS HEP-NOS I-NOS Hepatocytes Oxide Synthase Peptidyl-cysteine S-nitrosylase NOS2; NOS2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of NOS2. No significant cross-reactivity or interference between NOS2 and analogues was observed.
• Sequence Length: 1147

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.59ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NOS2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NOS2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOS2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOS2 in Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOS2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NOS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NOS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NOS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148298711
• NCBI GeneID: 24599
• NCBI Accession #: NP_036743.3
• NCBI GenBank Nucleotide #: NM_012611.3
• UniProt Accession #: Q06518; O35765; O35766; O60591; O60604; P97774; Q63267; Q64005; Q64558
• Molecular Weight: 130,628 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2
• NCBI Official Symbol: Nos2
• NCBI Official Synonym Symbols: iNos; Nos2a
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inducible NOS; iNOS

NCBI Description

cytokine-inducible enzmyme involved in nitric oxide (NO) production [RGD, Feb 2006]

Uniprot Description

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.