Rabbit anti-Human LEO1 Polyclonal Antibody | anti-LEO1 antibody

LEO1, NT (RDL, RNA Polymerase-associated Protein LEO1, Replicative Senescence Down-regulated leo1-like Protein, RDL) (APC)

Cat. Num. AAA6484856

• Gene Names: LEO1; RDL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: LEO1; Polyclonal Antibody; NT (RDL; RNA Polymerase-associated Protein LEO1; Replicative Senescence Down-regulated leo1-like Protein; RDL) (APC); anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LEO1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-LEO1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:250; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human LEO1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Leo1 Antibody (N-term) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-LEO1 antibody

Antibodies; Enzymes; Polymerase

References

1. Zhao, L., FASEB J. 19(6), 521-532 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 20270337
• NCBI GeneID: 123169
• NCBI Accession #: NP_620147
• NCBI GenBank Nucleotide #: NM_138792
• UniProt Accession #: Q8WVC0; Q96N99
• Molecular Weight: 68,004 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1 isoform 1
• NCBI Official Synonym Full Names: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: LEO1
• NCBI Official Synonym Symbols: RDL
• NCBI Protein Information: RNA polymerase-associated protein LEO1; replicative senescence down-regulated leo1-like protein
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: LEO1
• UniProt Synonym Gene Names: RDL
• UniProt Entry Name: LEO1_HUMAN

Uniprot Description

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding

Biological Process: histone monoubiquitination; Wnt receptor signaling pathway; negative regulation of myeloid cell differentiation; endodermal cell fate commitment; transcription, DNA-dependent; positive regulation of RNA elongation from RNA polymerase II promoter; stem cell maintenance; histone H2B ubiquitination; positive regulation of mRNA 3'-end processing; mRNA polyadenylation; positive regulation of transcription from RNA polymerase II promoter