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Western Blot (WB) (Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Rabbit anti-Human, Mouse LEMD1 Polyclonal Antibody | anti-LEMD1 antibody

LEMD1 Antibody

Gene Names
LEMD1; CT50; LEMP-1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
LEMD1 Antibody is affinity chromatography purified via peptide column.
Synonyms
LEMD1; Polyclonal Antibody; LEMD1 Antibody; CT50; LEMP-1; LEM domain-containing protein 1; LEM domain protein 1; LEM domain containing 1; anti-LEMD1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
At least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3.
Purity/Purification
LEMD1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; LEMD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
181
Applicable Applications for anti-LEMD1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Optimal dilutions for each application to be determined by the researcher.
Conjugate
Unconjugated
Immunogen
LEMD1 antibody was raised against a 19 amino acid synthetic peptide near the center of human LEMD1.
Positive Control
MBS151677 - A20 Cell Lysate
Preparation and Storage
LEMD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-LEMD1 antibody
LEMD1 Antibody: LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
7,503 Da
NCBI Official Full Name
LEM domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
LEM domain containing 1
NCBI Official Symbol
LEMD1
NCBI Official Synonym Symbols
CT50; LEMP-1
NCBI Protein Information
LEM domain-containing protein 1; LEM domain protein 1; cancer/testis antigen 50
UniProt Protein Name
LEM domain-containing protein 1
UniProt Gene Name
LEMD1
UniProt Synonym Gene Names
CT50; LEMP-1
UniProt Entry Name
LEMD1_HUMAN

Uniprot Description

LEMD1: 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: integral to membrane

Research Articles on LEMD1

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Product Notes

The LEMD1 lemd1 (Catalog #AAA151350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LEMD1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LEMD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the LEMD1 lemd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEMD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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