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COVID 19 3CLpro/3C-like protease-His & AVI Coronavirus Active Protein | COVID-19 active protein
SARS-CoV-2 (2019-nCoV) 3CLpro/3C-like protease-His & AVI Recombinant Protein
Purity
>90% as determined by SDS-PAGE
Synonyms
COVID 19 3CLpro/3C-like protease-His & AVI Coronavirus; SARS-CoV-2 (2019-nCoV) 3CLpro/3C-like protease-His & AVI Recombinant Protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; 3CLpro/3C-like protease-His & AVI; COVID-19 active protein
Host
Baculovirus-Insect Cells
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Ser3264-Gln3569
Species
SARS-CoV-2
Activity
Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans. The specific activity is >3 pmols/min/ug
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Met
Tag
N-His & AVI
Protein Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) 3CLpro (YP_009725295.1)(Ser3264-Gln3569) was expressed with a N-terminus polyhistide tag and AVI tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
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Related Product Information for COVID-19 active protein
Background: 3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.
References
Tomonari Muramatsu,et al.Autoprocessing mechanism of severe acute respiratory syndrome coronavirus 3C-like protease (SARS-CoV 3CLpro) from its polyproteins.FEBS Journal.2013
Ziebuhr J,Molecular biology of severe acute respiratory syndrome coronavirus. Curr Opin Microbiol.2004
Yang H, et al. The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. Proc Natl Acad Sci USA.2003
Ziebuhr J,Molecular biology of severe acute respiratory syndrome coronavirus. Curr Opin Microbiol.2004
Yang H, et al. The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. Proc Natl Acad Sci USA.2003
NCBI and Uniprot Product Information
Molecular Weight
The recombinant heterodimer of SARS-CoV-2 (2019-nCoV) 3CLpro consists of 332 amino acids and has a calculated molecular mass of 37.12kDa.
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Product Notes
The COVID-19 (Catalog #AAA8122428) is an Active Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Ser3264-Gl n3569. It is sometimes possible for the material contained within the vial of "COVID 19 3CLpro/3C-like protease-His & AVI Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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