Western Blot (WB)
(Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Chicken SIK3 Polyclonal Antibody | anti-SIK3 antibody
SIK3 Antibody
Gene Names
SIK3; L19; QSK; SIK-3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
SIK3 Antibody is affinity chromatography purified via peptide column.
Synonyms
SIK3; Polyclonal Antibody; SIK3 Antibody; L19; QSK; SIK-3; KIAA0999; Salt-inducible kinase 3; SIK family kinase 3; anti-SIK3 antibody
Host
Chicken
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
At least three isoforms of SIK3 are known to exist; this antibody will detect two isoforms. SIK3 antibody is predicted to not cross-react with SIK1 and SIK2.
Purity/Purification
SIK3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1263
Applicable Applications for anti-SIK3 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
SIK3 antibody can be used for detection of SIK3 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3.
Buffer
SIK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SIK3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunofluorescence (IF)
(Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.)
Related Product Information for anti-SIK3 antibody
SIK3 Antibody: Salt-inducible kinase family (SIKs) proteins are thought to have a role in steroidogenesis, adipogenesis or regulation of tumor malignancy. Three members (SIK1, SIK2 and SIK3) in the SIK family have been identified thus far. Their kinase domain sequences are closely homologous to those of AMP-activated protein kinase (AMPK). SIK3 can be phosphorylated by a tumor-suppressor kinase LKB1. It is highly and preferentially expressed in ovarian tumors but not in adenomyosis and leiomyoma and may be a potential marker for ovarian cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,715 Da
NCBI Official Full Name
serine/threonine-protein kinase SIK3
NCBI Official Synonym Full Names
SIK family kinase 3
NCBI Official Symbol
SIK3
NCBI Official Synonym Symbols
L19; QSK; SIK-3
NCBI Protein Information
serine/threonine-protein kinase SIK3; salt-inducible kinase 3; serine/threonine-protein kinase QSK
UniProt Protein Name
Serine/threonine-protein kinase SIK3
Protein Family
UniProt Gene Name
SIK3
UniProt Synonym Gene Names
KIAA0999; QSK; SIK-3
UniProt Entry Name
SIK3_HUMAN
Research Articles on SIK3
Similar Products
Product Notes
The SIK3 sik3 (Catalog #AAA150059) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The SIK3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). SIK3 antibody can be used for detection of SIK3 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SIK3 sik3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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