Western Blot (WB)
(Anti-Lamp3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:48 kDa)
Rabbit anti-Mouse LAMP3/CD208 Polyclonal Antibody | anti-LAMP3 antibody
Anti-LAMP3/CD208 Antibody, Rabbit Polyclonal
Gene Names
Lamp3; LAMP; Cd208; DCLAMP; TSC403; DC-LAMP; 1200002D17Rik
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
LAMP3/CD208; Polyclonal Antibody; Anti-LAMP3/CD208 Antibody; Rabbit Polyclonal; LAMP3 Antibody; Rabbit PAb; Antigen Affinity Purified; lysosomal associated membrane protein 3; Anti-1200002D17Rik Antibody; Anti-Cd208 Antibody; Anti-DC-LAMP Antibody; Anti-DCLAMP Antibody; Anti-LAMP Antibody; Anti-TSC403 Antibody; anti-LAMP3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse LAMP3/CD208
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-LAMP3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IHC-P: 1:200-1:1000
Immunogen
A synthetic peptide corresponding to the center region of the Mouse LAMP3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse LAMP3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-Lamp3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:48 kDa)
Western Blot (WB)
(Anti-Lamp3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:48 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse Lamp3 in mouse lung with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse Lamp3 in mouse lung with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-LAMP3 antibody
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in the activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
References
Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,129 Da
NCBI Official Full Name
lysosome-associated membrane glycoprotein 3
NCBI Official Synonym Full Names
lysosomal-associated membrane protein 3
NCBI Official Symbol
Lamp3
NCBI Official Synonym Symbols
LAMP; Cd208; DCLAMP; TSC403; DC-LAMP; 1200002D17Rik
NCBI Protein Information
lysosome-associated membrane glycoprotein 3; LAMP-3; DC LAMP; DC-lysosome-associated membrane glycoprotein; dendritic cells-lysosome associated membrane glycoprotein
UniProt Protein Name
Lysosome-associated membrane glycoprotein 3
Protein Family
UniProt Gene Name
Lamp3
UniProt Synonym Gene Names
LAMP-3; Lysosomal-associated membrane protein 3; DC LAMP
UniProt Entry Name
LAMP3_MOUSE
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Product Notes
The LAMP3 lamp3 (Catalog #AAA8101688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LAMP3/CD208 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LAMP3/CD208 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:200-1:1000. Researchers should empirically determine the suitability of the LAMP3 lamp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAMP3/CD208, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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