Immunofluorescence (IF)
(Immunofluorescence staining of Human LAMP3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LAMP3 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and countstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Rabbit anti-Human LAMP3/CD208 Monoclonal Antibody | anti-LAMP3 antibody
Recombinant Anti-LAMP3/CD208 Antibody, Rabbit Monoclonal
Gene Names
LAMP3; LAMP; CD208; DCLAMP; LAMP-3; TSC403; DC LAMP; DC-LAMP
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
LAMP3/CD208; Monoclonal Antibody; Recombinant Anti-LAMP3/CD208 Antibody; Rabbit Monoclonal; CD208/DC-LAMP/LAMP3 Antibody; Rabbit MAb; lysosomal associated membrane protein 3; Anti-CD208 Antibody; Anti-DC LAMP Antibody; Anti-DC-LAMP Antibody; Anti-DCLAMP Antibody; Anti-LAMP Antibody; Anti-LAMP-3 Antibody; Anti-TSC403 Antibody; anti-LAMP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
007
Specificity
Human CD208/DC-LAMP/LAMP3
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LAMP3 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD208/DC-LAMP/LAMP3 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD208/DC-LAMP/LAMP3 (rh CD208/DC-LAMP/LAMP3).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of Human LAMP3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LAMP3 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and countstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human LAMP3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LAMP3 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and countstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Related Product Information for anti-LAMP3 antibody
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in the activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
References
Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,346 Da
NCBI Official Full Name
lysosome-associated membrane glycoprotein 3
NCBI Official Synonym Full Names
lysosomal-associated membrane protein 3
NCBI Official Symbol
LAMP3
NCBI Official Synonym Symbols
LAMP; CD208; DCLAMP; LAMP-3; TSC403; DC LAMP; DC-LAMP
NCBI Protein Information
lysosome-associated membrane glycoprotein 3; DC-lysosome-associated membrane glycoprotein
UniProt Protein Name
Lysosome-associated membrane glycoprotein 3
Protein Family
UniProt Gene Name
LAMP3
UniProt Synonym Gene Names
DCLAMP; TSC403; LAMP-3; Lysosomal-associated membrane protein 3; DC LAMP
UniProt Entry Name
LAMP3_HUMAN
NCBI Description
Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM, Dec 2010]
Uniprot Description
LAMP3: Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. Belongs to the LAMP family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q26.3-q27
Cellular Component: lysosomal membrane; integral to membrane
Biological Process: cell proliferation; immune system process
Research Articles on LAMP3
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Product Notes
The LAMP3 lamp3 (Catalog #AAA8103125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-LAMP3/CD208 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAMP3/CD208 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the LAMP3 lamp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAMP3/CD208, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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