LAMP3/CD208 Recombinant Protein | LAMP3 recombinant protein

LAMP3/CD208 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120334

• Gene Names: LAMP3; LAMP; CD208; DCLAMP; LAMP-3; TSC403; DC LAMP; DC-LAMP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LAMP3/CD208; LAMP3/CD208 Protein; Human; Recombinant (His Tag); Human CD208/LAMP3/DC-LAMP Protein (His Tag); CD208 Protein; DC LAMP Protein; DC-LAMP Protein; DCLAMP Protein; LAMP Protein; LAMP-3 Protein; TSC403 Protein; lysosomal associated membrane protein 3; LAMP3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys28-Thr381

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human LAMP3 (NP_055213.2)(Lys 28-Thr 381) with a carboxy-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LAMP3 recombinant protein

Background: Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in the activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

References

Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.

NCBI and Uniprot Product Information

• NCBI GI #: 38455385
• NCBI GeneID: 27074
• NCBI Accession #: NP_055213.2
• NCBI GenBank Nucleotide #: NM_014398.3
• UniProt Accession #: Q9UQV4; O94781; Q8NEC8; D3DNS4
• Molecular Weight: 44,346 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 3
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 3
• NCBI Official Symbol: LAMP3
• NCBI Official Synonym Symbols: LAMP; CD208; DCLAMP; LAMP-3; TSC403; DC LAMP; DC-LAMP
• NCBI Protein Information: lysosome-associated membrane glycoprotein 3; DC-lysosome-associated membrane glycoprotein
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 3
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: LAMP3
• UniProt Synonym Gene Names: DCLAMP; TSC403; LAMP-3; Lysosomal-associated membrane protein 3; DC LAMP
• UniProt Entry Name: LAMP3_HUMAN

NCBI Description

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM, Dec 2010]

Uniprot Description

LAMP3: Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. Belongs to the LAMP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26.3-q27

Cellular Component: lysosomal membrane; integral to membrane

Biological Process: cell proliferation; immune system process