CD208 / LAMP3 / DC-LAMP recombinant protein

Recombinant Human CD208 / LAMP3 / DC-LAMP Protein (His tag)

Cat. Num. AAA2546201

• Gene Names: LAMP3; LAMP; CD208; DCLAMP; LAMP-3; TSC403; DC LAMP; DC-LAMP
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD208 / LAMP3 / DC-LAMP; Recombinant Human CD208 / LAMP3 / DC-LAMP Protein (His tag); CD208; DC-LAMP; DCLAMP; LAMP; TSC403; CD208 / LAMP3 / DC-LAMP recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 416

• Application Notes: The secreted recombinant human LAMP3 consists of 365 amino acids with the predicted molecular mass of 39 kDa. As a result of glycosylation, rh LAMP3 migrates as an approximately 70-90 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Lys 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD208 / LAMP3 / DC-LAMP recombinant protein

Background: Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

Description: A DNA sequence encoding the mature form of human LAMP3 (NP_055213.2) (Lys 28-Thr 381) with a carboxy-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 38455385
• NCBI GeneID: 27074
• NCBI Accession #: NP_055213.2
• NCBI GenBank Nucleotide #: NM_014398.3
• UniProt Accession #: Q9UQV4; O94781; Q8NEC8; D3DNS4
• Molecular Weight: 44,346 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 3
• NCBI Official Synonym Full Names: lysosomal associated membrane protein 3
• NCBI Official Symbol: LAMP3
• NCBI Official Synonym Symbols: LAMP; CD208; DCLAMP; LAMP-3; TSC403; DC LAMP; DC-LAMP
• NCBI Protein Information: lysosome-associated membrane glycoprotein 3
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 3
• UniProt Gene Name: LAMP3
• UniProt Synonym Gene Names: DCLAMP; TSC403; LAMP-3; Lysosomal-associated membrane protein 3; DC LAMP

NCBI Description

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM, Dec 2010]

Uniprot Description

LAMP3: plays a role in dendritic cell function and in adaptive immunity. Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. Belongs to the LAMP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: early endosome; perinuclear region of cytoplasm; plasma membrane; vesicle

Biological Process: entry of virus into host cell; negative regulation of caspase activity; regulation of autophagy